在搅拌罐反应器中，产黄青霉分批培养产生的寡糖提高青霉素产量。

Enhanced penicillin production by oligosaccharides from batch cultures of Penicillium chrysogenum in stirred-tank reactors.

作者信息

Ariyo B, Tamerler C, Bucke C, Keshavarz T

机构信息

Biotechnology Department, University of Westminster, London, UK.

出版信息

FEMS Microbiol Lett. 1998 Sep 1;166(1):165-70. doi: 10.1111/j.1574-6968.1998.tb13198.x.

DOI:10.1111/j.1574-6968.1998.tb13198.x

PMID:9776634

Abstract

Alginate and galactomannan-derived oligosaccharides enhanced the production of penicillin G when added to stirred tank reactor cultures of Penicillium chrysogenum. The addition of oligomannuronate and oligoguluronate blocks increased penicillin G yield by 47% and 49%, respectively. The effect of mannan oligosaccharides was found to be more pronounced with 69% higher yield than the control cultures. The maximum increase in the average specific productivity of the oligosaccharide augmented cultures was 55% after addition of mannan oligosaccharides. In addition, a difference was observed in all cases in the accumulation pattern of the intermediate of penicillin biosynthesis, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine.

摘要

当将藻酸盐和半乳甘露聚糖衍生的低聚糖添加到产黄青霉的搅拌罐反应器培养物中时，可提高青霉素G的产量。添加聚甘露糖醛酸和聚古罗糖醛酸片段分别使青霉素G的产量提高了47%和49%。发现甘露寡糖的效果更显著，产量比对照培养物高69%。添加甘露寡糖后，低聚糖增强培养物的平均比生产率的最大增幅为55%。此外，在所有情况下，青霉素生物合成中间体δ-(L-α-氨基己二酰基)-L-半胱氨酰-D-缬氨酸的积累模式均存在差异。