一种在巴拿马感染番茄的新型粉虱传播双分体双生病毒的分子特征

Molecular characterization of a new whitefly-transmissible bipartite geminivirus infecting tomato in Panama.

作者信息

Engel M, Fernández O, Jeske H, Frischmuth T

机构信息

Biologisches Institut, Lehrstuhl für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Germany.

出版信息

J Gen Virol. 1998 Oct;79 ( Pt 10):2313-7. doi: 10.1099/0022-1317-79-10-2313.

DOI:10.1099/0022-1317-79-10-2313

PMID:9780034

Abstract

The nucleotide sequence of infectious clones of a tomato-infecting geminivirus from Panama [named tomato leaf curl virus (ToLCV-Pan) because of symptoms produced in infected tomato (plant stunting and mild leaf curling)] was determined. ToLCV-Pan has a bipartite genome (DNAs A and B) and computer analysis showed that the genome resembles that of other bipartite, whitefly-transmitted geminiviruses. DNA A (2584 nt) and B (2542 nt) have little sequence homology other than within the common region. ToLCV-Pan clones were introduced into Lycopersicon esculentum and infected plants developed the same symptoms as naturally infected tomatoes. Homology analysis of DNA A and B showed that ToLCV-Pan is most closely related to potato yellow mosaic virus (PYMV) from Venezuela. Pseudorecombination between ToLCV-Pan and PYMV did not give viable pseudorecombinant viruses. However, in some plants infected with the pseudorecombinant virus produced by ToLCV-Pan DNA A and PYMV DNA B, systemic movement of ToLCV-Pan DNA A was observed.

摘要

测定了一种来自巴拿马的侵染番茄的双生病毒（因感染番茄产生的症状——植株矮化和轻度叶片卷曲而命名为番茄卷叶病毒（ToLCV-Pan））感染性克隆的核苷酸序列。ToLCV-Pan具有双分体基因组（DNA A和B），计算机分析表明该基因组与其他双分体、粉虱传播的双生病毒的基因组相似。DNA A（2584个核苷酸）和B（2542个核苷酸）除了在共同区域内，几乎没有序列同源性。将ToLCV-Pan克隆导入番茄中，被感染的植株出现了与自然感染番茄相同的症状。DNA A和B的同源性分析表明，ToLCV-Pan与来自委内瑞拉的马铃薯黄花叶病毒（PYMV）关系最为密切。ToLCV-Pan和PYMV之间的假重组未产生可存活的假重组病毒。然而，在一些被ToLCV-Pan DNA A和PYMV DNA B产生的假重组病毒感染的植株中，观察到了ToLCV-Pan DNA A的系统移动。