小鼠肝炎病毒核衣壳蛋白作为病毒mRNA的翻译效应因子
Mouse hepatitis virus nucleocapsid protein as a translational effector of viral mRNAs.
作者信息
Tahara S M, Dietlin T A, Nelson G W, Stohlman S A, Manno D J
机构信息
Department of Molecular Microbiology and Immunology, USC School of Medicine, Los Angeles 90033, USA.
出版信息
Adv Exp Med Biol. 1998;440:313-8. doi: 10.1007/978-1-4615-5331-1_41.
The mouse hepatitis virus (MHV) nucleocapsid protein stimulated translation of a chimeric reporter mRNA containing an intact MHV 5'-untranslated region and the chloramphenicol acetyltransferase (CAT) coding region. The nucleocapsid protein binds specifically the tandemly repeated-UCYAA- of the MHV leader. This RNA sequence is the same as the intergenic motif found in the genome RNA. Preferential translation of viral mRNA in MHV infected cells is stimulated in part by this interaction and represents a specific, positive translational control mechanism employed by coronaviruses.
小鼠肝炎病毒（MHV）核衣壳蛋白可刺激一种嵌合报告基因mRNA的翻译，该mRNA包含完整的MHV 5'-非翻译区和氯霉素乙酰转移酶（CAT）编码区。核衣壳蛋白特异性结合MHV前导序列串联重复的-UCYAA-。该RNA序列与在基因组RNA中发现的基因间基序相同。这种相互作用部分刺激了MHV感染细胞中病毒mRNA的优先翻译，这代表了冠状病毒采用的一种特定的、正向翻译控制机制。
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