Kudchodkar B J, Horlick L, O'Neil J B
J Nutr. 1976 Nov;106(11):1629-36. doi: 10.1093/jn/106.11.1629.
Studies were undertaken to determine the dietary beta-sitosterol absorption in laying hens and its incorporation into the egg. Hens were divided into four groups and fed a commercial low-fat laying diet. Group 1 served as controls; the diet of group 2 was supplemented with 10% corn oil; group 3 with 4% plant sterols (emulsion in carboxymethylcellulose); group 4 with 10% corn oil and 4% plant sterols. The daily beta-sitosterol intake of hens in groups 1, 2, 3 and 4 was 0.036 g 0.095 g, 2 g and 2.56 g, respectively. After consuming the diets for 30 to 40 days, cholesterol and beta-sitosterol contents of the eggs were estimated by gas liquid chromatographic and mass spectrometric methods. Feeding corn oil with and without plant sterols increased cholesterol content of the eggs, while feeding plant sterols alone had no effect on egg cholesterol levels. The beta-sitosterol content of eggs from groups 1 and 2 was similar (60-66 mug/g yolk) and feeding plant sterols increased the beta-sitosterol content to a very small extent (82-90 mug/g yolk). Studies on the dietary beta-sitosterol and cholesterol absorption indicated that there was very little absorption of beta-sitosterol. In control hens, beta-sitosterol absorption was about 7% compared to 81% cholesterol absorption. Plant sterols feeding did not affect the beta-sitosterol absorption but reduced cholesterol absorption by 40%. Feeding corn oil alone did not affect cholesterol absorption. Failure to detect any significant amount of beta-sitosterol in these hens. At the peak radioactivity deposition in the eggs, only 2% of the absorbed beta-sitosterol radioactivity was found in the eggs of group 4 compared to 5% found in group 1, while cholesterol radiioactivity was nearly 4 to 5 times in both groups. These data suggested that laying hens synthesized specific lipoproteins for deposition in yolk.
开展了多项研究以确定蛋鸡日粮中β-谷甾醇的吸收情况及其在鸡蛋中的掺入情况。将母鸡分为四组，饲喂市售低脂蛋鸡日粮。第1组作为对照组；第2组日粮添加10%玉米油；第3组添加4%植物甾醇（羧甲基纤维素乳液）；第4组添加10%玉米油和4%植物甾醇。第1、2、3和4组母鸡的每日β-谷甾醇摄入量分别为0.036克、0.095克、2克和2.56克。在采食日粮30至40天后，采用气相色谱和质谱法测定鸡蛋中的胆固醇和β-谷甾醇含量。饲喂添加和未添加植物甾醇的玉米油均会增加鸡蛋中的胆固醇含量，而单独饲喂植物甾醇对鸡蛋胆固醇水平没有影响。第1组和第2组鸡蛋的β-谷甾醇含量相似（60 - 66微克/克蛋黄），饲喂植物甾醇仅使β-谷甾醇含量略有增加（82 - 90微克/克蛋黄）。日粮中β-谷甾醇和胆固醇吸收的研究表明，β-谷甾醇的吸收极少。在对照母鸡中，β-谷甾醇的吸收约为7%，而胆固醇的吸收为81%。饲喂植物甾醇不影响β-谷甾醇的吸收，但可使胆固醇吸收降低40%。单独饲喂玉米油不影响胆固醇吸收。在这些母鸡中未检测到任何显著量的β-谷甾醇。在鸡蛋中放射性沉积峰值时，第4组鸡蛋中仅2%的吸收β-谷甾醇放射性被发现，而第1组为5%，同时两组中胆固醇放射性几乎是4至5倍。这些数据表明，蛋鸡合成了特定的脂蛋白用于沉积在蛋黄中。
