Krakauer M, Schaldemose Nielsen H, Jensen J, Sellebjerg F
Department of Neurology, Glostrup Hospital, University of Copenhagen, Denmark.
Acta Neurol Scand. 1998 Sep;98(3):161-5. doi: 10.1111/j.1600-0404.1998.tb07287.x.
The detection of oligoclonal immunoglobulin free light chains (FLC) in the diagnosis of multiple sclerosis (MS) was compared to IgG isoelectric focusing.
Cerebrospinal fluid and serum samples from 69 patients with possible first attacks of MS, 50 patients with clinically definite MS (CDMS), and 118 patients with other neurological diseases (OND) were analyzed. IgG and FLC oligoclonal bands were detected by isoelectric focusing and immunoperoxidase staining.
Intrathecal synthesis of IgG, kappa FLC, and lambda FLC oligoclonal bands, respectively, was seen in 92%, 92%, and 86% of MS patients; in 61%, 62%, and 64% of patients with possible first attacks of MS; and in 3%, 3%, and 8% of the patients with OND. In control patients without IgG synthesis intrathecal lambda FLC synthesis was more common than kappa FLC synthesis (P=0.03).
Kappa FLC detection proved as useful as IgG analysis for the laboratory diagnosis of MS whereas the presence of intrathecal lambda FLC synthesis was less specific.
比较寡克隆免疫球蛋白游离轻链（FLC）检测与IgG等电聚焦在多发性硬化症（MS）诊断中的作用。
对69例可能首次发作MS的患者、50例临床确诊MS（CDMS）患者及118例其他神经系统疾病（OND）患者的脑脊液和血清样本进行分析。通过等电聚焦和免疫过氧化物酶染色检测IgG和FLC寡克隆带。
MS患者中，IgG、κFLC和λFLC寡克隆带鞘内合成率分别为92%、92%和86%；可能首次发作MS的患者中分别为61%、62%和64%；OND患者中分别为3%、3%和8%。在无IgG合成的对照患者中，鞘内λFLC合成比κFLC合成更常见（P = 0.03）。
κFLC检测在MS实验室诊断中与IgG分析同样有用，而鞘内λFLC合成的存在特异性较低。
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