Robinson E J, Neal D E, Collins A T
Smith and Nephew Group Research Centre, Heslington, York, United Kingdom.
Prostate. 1998 Nov 1;37(3):149-60. doi: 10.1002/(sici)1097-0045(19981101)37:3<149::aid-pros4>3.0.co;2-e.
Human prostatic epithelium consists mainly of basal and secretory luminal cells: the origin of these phenotypes from a common stem cell, within the basal compartment, has been proposed but not yet demonstrated.
Analyses by light and electron microscopy, immunocytochemistry, and flow cytometry were used to determine lineage. The criteria for identifying the different phenotypes were characteristic morphology, and organization and expression of luminal- and basal-specific markers.
After organoids attached, outgrowths appeared with cells maintaining close cell-to-cell associations. The dividing cell compartment contained a subpopulation of cells with stem-cell characteristics and a major population that may correspond to amplifying cells. The characteristics of the stem-cell phenotype included reactivity with antibodies CKbasal, CK14, and Ki67. The amplifying cells were characterized as an intermediate phenotype between basal and luminal, as reactivity was demonstrated with CKbasal, CK14, and CK18. As outgrowths eventually merged, multilayering was apparent and cells on the uppermost layer had numerous secretory vacuoles and reacted strongly with antibodies CK18 and CK19, androgen receptor, and prostate-specific antigen, which is characteristic of secretory luminal cells in vivo. In passaged cultures, loss of reactivity with CKbasal was detected; we postulate that this population contains the stem-cell fraction.
These findings demonstrate that basal and luminal cells are of the same lineage and are derived from a common stem cell. Moreover, the progenitor stem cells reside within the basal compartment.
人前列腺上皮主要由基底细胞和分泌性管腔细胞组成：已有人提出这些表型起源于基底区室中的一个共同干细胞，但尚未得到证实。
采用光镜和电镜分析、免疫细胞化学和流式细胞术来确定细胞谱系。鉴定不同表型的标准是特征性形态以及管腔特异性和基底特异性标志物的组织和表达。
类器官附着后，出现了细胞间保持紧密联系的细胞生长物。分裂细胞区室包含具有干细胞特征的细胞亚群和可能对应于增殖细胞的主要群体。干细胞表型的特征包括与抗体CKbasal、CK14和Ki67反应。增殖细胞的特征是介于基底细胞和管腔细胞之间的中间表型，因为它们与CKbasal、CK14和CK18反应。随着细胞生长物最终融合，明显出现多层结构，最上层的细胞有许多分泌泡，并与抗体CK18、CK19、雄激素受体和前列腺特异性抗原强烈反应，这是体内分泌性管腔细胞的特征。在传代培养中，检测到与CKbasal反应性丧失；我们推测该群体包含干细胞部分。
这些发现表明基底细胞和管腔细胞属于同一谱系，并且起源于一个共同的干细胞。此外，祖干细胞存在于基底区室中。
