Ohyama A, Nishimura S, Hirai M
National Research Institute of Vegetables, Ornamental Plants and Tea (NIVOT), Mie, Japan.
Genes Genet Syst. 1998 Jun;73(3):149-57. doi: 10.1266/ggs.73.149.
A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was cloned from wounded leaves of tomato (Lycopersicon esculentum). The encoded protein had a basic isoelectric point and strong similarity to the amino acid sequences of plant cell wall-bound invertases. The conserved sequence WECPD that is found in all plant cell wall-bound invertases was also found in the deduced protein. These results suggested that Wiv-1 encoded a cell wall-bound acid invertase of tomato. Wounding increased the levels of mRNAs for soluble and cell wall-bound invertases and the activities of these invertases in leaves of L. esculentum and of a related species, L. peruvianum. The induction of Aiv-1 mRNA for the soluble enzyme in wounded leaves was not very strong, while that of Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1 mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA continued to rise for up to 96 h. These findings suggested that the genes for the two isozymes responded independently to wounding. The levels in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculentum than in L. peruvianum. Possible roles of cell wall-bound acid invertase in wound response and in developing plant are discussed.
从番茄（Lycopersicon esculentum）受伤叶片中克隆到一种酸性转化酶（EC 3.2.1.26）同工酶的cDNA，即Wiv-1。该编码蛋白具有碱性等电点，与植物细胞壁结合型转化酶的氨基酸序列有很强的相似性。在推导的蛋白中也发现了所有植物细胞壁结合型转化酶中都存在的保守序列WECPD。这些结果表明Wiv-1编码番茄的一种细胞壁结合型酸性转化酶。创伤增加了番茄（L. esculentum）及其近缘种秘鲁番茄（L. peruvianum）叶片中可溶性和细胞壁结合型转化酶的mRNA水平以及这些转化酶的活性。创伤叶片中可溶性酶Aiv-1 mRNA的诱导不太强烈，而细胞壁结合酶Wiv-1 mRNA的诱导则很显著。Aiv-1 mRNA水平在创伤后48小时达到最高，而Wiv-1 mRNA水平在长达96小时内持续上升。这些发现表明这两种同工酶的基因对创伤的反应是独立的。番茄（L. esculentum）中Aiv-1和Wiv-1 mRNA在各个器官中的水平高于秘鲁番茄（L. peruvianum）。文中讨论了细胞壁结合型酸性转化酶在创伤反应和植物发育中的可能作用。
