Fernàndez J E, Deaglio S, Donati D, Beusan I S, Corno F, Aranega A, Forni M, Falini B, Malavasi F
Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Italy.
J Biol Regul Homeost Agents. 1998 Jul-Sep;12(3):81-91.
Human CD38 is a 45 kD ectoenzyme endowed with ADP-ribosyl cyclase and hydrolase activities. The molecule plays a central role in lymphocyte activation, proliferation and selectin-type adhesion with endothelial cells (HEC). A HEC surface molecule displaying all the features of a CD38L has been identified by means of a mAb (Moon-1), able to block CD38-mediated adhesion processes. The 130 kD molecule recognized by Moon-1 is CD31, a member of the Ig superfamily. This paper reports on the analysis of the surface expression of CD38 and CD38L in various human tissues of adult origin and compared in some instances to the fetal (9-14 weeks) counterparts. This was achieved by means of immunohistochemical techniques and analysis of purified cell populations. Among the specimens analyzed, CD38 is expressed by a vast array of cells of lymphatic origin as well as by the skeletal and cardiac muscle fibers, the bronchi (epithelial cells), the parotid gland (ductal epithelial cells) and hepatic sinusoids. On the contrary, CD31 proved constantly expressed by HEC at high levels, independent of the organ or tissue analyzed or of the kind of vessel. Other cells expressing CD38L were found in the lymphoid compartment (follicle mantle B cells and plasma cells), in the lungs (alveolar ducts, alveoli and lymphatic vessels) and in the kidney (glomerular cells). Interestingly, no fetal organ or tissue ever expressed CD38 and its ligand. The above results were enriched by the analysis of the expression of the two molecules on purified populations including mononuclear cells from the lamina propria of the gastro-intestinal tract and broncho-alveolar lavage lymphocytes.
人CD38是一种45kD的胞外酶，具有ADP - 核糖基环化酶和水解酶活性。该分子在淋巴细胞活化、增殖以及与内皮细胞（HEC）的选择素型黏附中起核心作用。通过一种单克隆抗体（Moon - 1）鉴定出一种显示出CD38L所有特征的HEC表面分子，该抗体能够阻断CD38介导的黏附过程。Moon - 1识别的130kD分子是CD31，它是免疫球蛋白超家族的成员。本文报道了对成年来源的各种人体组织中CD38和CD38L表面表达的分析，并在某些情况下与胎儿（9 - 14周）的相应组织进行了比较。这是通过免疫组织化学技术和对纯化细胞群体的分析实现的。在所分析的标本中，CD38由大量淋巴来源的细胞表达，以及骨骼肌和心肌纤维、支气管（上皮细胞）、腮腺（导管上皮细胞）和肝血窦。相反，事实证明CD31在HEC中持续高水平表达，与所分析的器官或组织以及血管类型无关。在淋巴区（滤泡套B细胞和浆细胞）、肺（肺泡管、肺泡和淋巴管）和肾（肾小球细胞）中发现了其他表达CD38L的细胞。有趣的是，没有胎儿器官或组织表达过CD38及其配体。通过对包括来自胃肠道固有层的单核细胞和支气管肺泡灌洗淋巴细胞在内的纯化群体上这两种分子表达的分析，丰富了上述结果。
