由包含Semliki森林病毒T辅助细胞表位和HIV B细胞表位的嵌合肽在BALB/c小鼠中诱导HIV-1 IIIb中和抗体

Induction of HIV-1 IIIb neutralizing antibodies in BALB/c mice by a chimaeric peptide consisting of a T-helper cell epitope of Semliki Forest virus and a B-cell epitope of HIV.

作者信息

Fernández I M, Golding H, Benaissa-Trouw B J, de Vos N M, Harmsen M, Nottet H S, Golding B, Puijk W C, Meloen R H, Snippe H, Kraaijeveld C A

机构信息

Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Hospital, Utrecht, The Netherlands.

出版信息

Vaccine. 1998 Dec;16(20):1936-40. doi: 10.1016/s0264-410x(98)00128-5.

DOI:10.1016/s0264-410x(98)00128-5

PMID:9796047

Abstract

A colinearly synthesized peptide consisting of a H-2d restricted T-helper cell epitope of Semliki Forest virus (SFV) and triple repeats of sequence GPGRAF, derived from the V3 domain of HIV-1 strains, was used to immunize BALB/c (H-2d) mice. Pepscan analysis of sera from peptide-immunized mice revealed that the chimaeric peptide GREKFTIRPHYGKEIGPGRAFGPGRAFGPGRAF contains three distinct antibody-reactive sequences GREKFTIR, PHYGKEI and GPGRAF. The chimaeric peptide evoked HIV-1 IIIb neutralizing antibodies in serum as measured in vitro by reduction of syncytia formation and reduction of p24 production as well. So, the T-helper cell epitope of SFV provided help to a small linear neutralization epitope of HIV-1 strains. Interestingly, the T-helper cell epitope alone might induce antibodies cross-reactive with HIV-1 IIIb specific peptide GPGRAFVTIGK which shows some homology (residues underlined) with the antibody-reactive sequence GREKTIR of SFV.

摘要

一种由塞姆利基森林病毒（SFV）的H-2d限制性辅助性T细胞表位和源自HIV-1毒株V3结构域的序列GPGRAF的三重重复序列组成的共线性合成肽，被用于免疫BALB/c（H-2d）小鼠。对肽免疫小鼠血清的肽扫描分析显示，嵌合肽GREKFTIRPHYGKEIGPGRAFGPGRAFGPGRAF包含三个不同的抗体反应序列GREKFTIR、PHYGKEI和GPGRAF。通过减少合胞体形成和降低p24产生在体外测量，嵌合肽在血清中诱发了HIV-1 IIIb中和抗体。因此，SFV的辅助性T细胞表位为HIV-1毒株的一个小线性中和表位提供了帮助。有趣的是，单独的辅助性T细胞表位可能诱导与HIV-1 IIIb特异性肽GPGRAFVTIGK交叉反应的抗体，该肽与SFV的抗体反应序列GREKTIR有一些同源性（下划线所示残基）。