Millar A A, Wrischer M, Kunst L
Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
Plant Cell. 1998 Nov;10(11):1889-902. doi: 10.1105/tpc.10.11.1889.
Transgenic Arabidopsis plants overexpressing the Arabidopsis FATTY ACID ELONGATION1 gene under the control of the 35S promoter from cauliflower mosaic virus accumulated very-long-chain fatty acids (VLCFAs) throughout the plant. In some transformants, C20 and C22 VLCFAs accounted for >30% of the total fatty acids, accumulating at the expense of C16 and C18 fatty acids. These C20 and C22 fatty acids were incorporated into all of the major membrane glycerolipid classes. Plants with a high VLCFA content displayed a dramatically altered morphology, which included the failure of flowering shoots to elongate, a modified spatial pattern of siliques, an altered floral phenotype, and a large accumulation of anthocyanins. In addition, these plants also exhibited a unique alteration of the chloroplast membrane structure. We discuss a possible role for VLCFAs in establishing the shape/curvature of the membranes, which in turn may affect the shape of the cell and ultimately that of the whole plant.
在花椰菜花叶病毒35S启动子控制下过表达拟南芥脂肪酸延长酶1基因的转基因拟南芥植株，在整个植株中积累了超长链脂肪酸（VLCFA）。在一些转化体中，C20和C22超长链脂肪酸占总脂肪酸的比例超过30%，其积累是以C16和C18脂肪酸为代价的。这些C20和C22脂肪酸被整合到所有主要的膜甘油脂类别中。具有高超长链脂肪酸含量的植株表现出显著改变的形态，包括花茎伸长受阻、角果空间模式改变、花表型改变以及花青素大量积累。此外，这些植株还表现出叶绿体膜结构的独特改变。我们讨论了超长链脂肪酸在建立膜的形状/曲率方面可能的作用，这反过来可能影响细胞的形状，最终影响整个植株的形状。
