Kalnenieks U, Galinina N, Bringer-Meyer S, Poole R K
Institute of Microbiology and Biotechnology, University of Latvia, Riga, Latvia.
FEMS Microbiol Lett. 1998 Nov 1;168(1):91-7. doi: 10.1111/j.1574-6968.1998.tb13260.x.
Respiratory chain composition of the ethanol-producing bacterium Zymomonas mobilis was studied. Its membrane D-lactate oxidase was characterised. With NADH, but not D-lactate as substrate, a cytochrome o-like component was seen in CO difference spectra. Chlorpromazine specifically inhibited reduction of cytochrome d, while myxothiazol eliminated the cytochrome o-like features in CO difference spectra. It is suggested that electrons from NADH are distributed between branches terminated by the cytochrome o-like component, cytochrome a, and cytochrome d. With D-lactate, electrons are transported to cytochrome a, or an unidentified CN(-)-sensitive oxidase, and cytochrome d.
对产乙醇细菌运动发酵单胞菌的呼吸链组成进行了研究。对其膜D-乳酸氧化酶进行了表征。以NADH而非D-乳酸为底物时，在CO差光谱中可见一种细胞色素o样成分。氯丙嗪特异性抑制细胞色素d的还原，而粘噻唑消除了CO差光谱中的细胞色素o样特征。表明来自NADH的电子分布在由细胞色素o样成分、细胞色素a和细胞色素d终止的分支之间。以D-乳酸为底物时，电子被转运至细胞色素a或一种未鉴定的对CN(-)敏感的氧化酶以及细胞色素d。
