Schumann G B, Lauenstein K, LeFever D, Henry J B
Am J Clin Pathol. 1976 Nov;66(5):823-30. doi: 10.1093/ajcp/66.5.823.
Several laboratory methods are currently available for the detection of barbiturates, but few are suitable for emergency use, The authors evaluated use of an ultraviolet spectrophotometric method as a screening procedure and compared the results with results obtained by other barbiturate methods (colorimetric, gas-liquid chromatographic, and immunochemical), Procedure time was approximately 40 minutes, sensitivity 0.2 mg/dl, precision (coefficient of variation) 5.4%, recovery 97.8% for phenobarbital, and there was good correlation with results obtained by gas-liquid chromatography and the EMIT system for phenobarbital. The authors recommend ultraviolet spectrophotometry as an excellent screening procedure for assessing the severity of barbiturate intoxication.
目前有几种实验室方法可用于检测巴比妥酸盐，但适合紧急使用的方法很少。作者评估了将紫外分光光度法用作筛查程序，并将结果与其他巴比妥酸盐检测方法（比色法、气液色谱法和免疫化学法）所得结果进行比较。检测过程约需40分钟，灵敏度为0.2mg/dl，精密度（变异系数）为5.4%，苯巴比妥回收率为97.8%，且与气液色谱法及苯巴比妥EMIT系统所得结果具有良好的相关性。作者推荐紫外分光光度法作为评估巴比妥酸盐中毒严重程度的一种出色的筛查程序。
