Tanaka S, Kunath T, Hadjantonakis A K, Nagy A, Rossant J
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
Science. 1998 Dec 11;282(5396):2072-5. doi: 10.1126/science.282.5396.2072.
The trophoblast cell lineage is essential for the survival of the mammalian embryo in utero. This lineage is specified before implantation into the uterus and is restricted to form the fetal portion of the placenta. A culture of mouse blastocysts or early postimplantation trophoblasts in the presence of fibroblast growth factor 4 (FGF4) permitted the isolation of permanent trophoblast stem cell lines. These cell lines differentiated to other trophoblast subtypes in vitro in the absence of FGF4 and exclusively contributed to the trophoblast lineage in vivo in chimeras.
滋养层细胞谱系对于哺乳动物胚胎在子宫内的存活至关重要。该谱系在植入子宫之前就已确定，并局限于形成胎盘的胎儿部分。在成纤维细胞生长因子4（FGF4）存在的情况下，对小鼠囊胚或植入后早期滋养层细胞进行培养，可以分离出永久性滋养层干细胞系。这些细胞系在没有FGF4的情况下，可在体外分化为其他滋养层亚型，并且在嵌合体中，在体内专门分化为滋养层谱系。
