维生素D和类视黄醇对犬骨肉瘤POS细胞凋亡的诱导作用。

Apoptosis induction of POS canine osteosarcoma cells by vitamin D and retinoids.

作者信息

Barroga E F, Kadosawa T, Asano K, Okumura M, Fujinaga T

机构信息

Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

出版信息

J Vet Med Sci. 1998 Nov;60(11):1269-72. doi: 10.1292/jvms.60.1269.

DOI:10.1292/jvms.60.1269

PMID:9853313

Abstract

Vitamin D3: 1-alpha, 25(OH)2D3 (calcitriol), 22-oxa-1,25(OH)2D3 (OCT), cholecalciferol (vitamin D3), and retinoids: all-trans retinoic acid (ATRA) and 9-cis retinoic acid, induced morphological changes in POS canine osteosarcoma cells into elongated, spindle or fibroblast like-shaped cells, and apoptotic like cell death characterized by cell shrinkage, condensation and margination of the nucleus for all drugs at 10(-6)M-10(-9)M after 72 to 120 hr culture. Apoptosis as shown by DNA laddering was induced at 48 hr by all drugs at 10(-6)M, 10(-7)M at 96 hr, 10(-8)M and 10(-9)M at 120 hr respectively. These vitamins are suggested to adjunct antineoplastic agents in canine osteosarcoma therapy by induction of apoptosis.

摘要

维生素D3：1-α，25-二羟维生素D3（骨化三醇）、22-氧杂-1，25-二羟维生素D3（OCT）、胆钙化醇（维生素D3），以及类视黄醇：全反式维甲酸（ATRA）和9-顺式维甲酸，在培养72至120小时后，10(-6)M - 10(-9)M的所有药物均可诱导犬骨肉瘤POS细胞发生形态学变化，转变为细长形、纺锤形或成纤维细胞样形状的细胞，并出现类似凋亡的细胞死亡，其特征为细胞收缩、细胞核凝聚和边缘化。DNA梯状条带显示，所有药物在10(-6)M时于48小时诱导凋亡，在10(-7)M时于96小时诱导凋亡，在10(-8)M和10(-9)M时于120小时分别诱导凋亡。这些维生素被认为可通过诱导凋亡辅助犬骨肉瘤治疗中的抗肿瘤药物。