Dumont Y, Jacques D, Bouchard P, Quirion R
Douglas Hospital Research Center, McGill University, Verdun, QC, Canada.
J Comp Neurol. 1998 Dec 21;402(3):372-84.
The respective distribution of neuropeptide Y Y1, Y2, Y4, and Y5 receptor subtypes was investigated in rodents (rat and mouse), guinea pig, and primates (marmoset and vervet monkeys and human) brains, representing three orders of mammals. [125I] [Leu31,Pro34]PYY (total Y1-like; Y1, Y4, and Y5) and [125I]PYY3-36 (total Y2-like; Y2 and possibly Y5) binding sites were discretely but similarly distributed in the rat and mouse brain, each having its unique pattern. In contrast, surprisingly low levels of [125I]PYY3-36 binding sites were found in the guinea pig brain including in the hippocampal formation. [125I][Leu31,Pro34]PYY/BIBP3226-insensitive binding sites (Y5-like) were found in different areas of the rat and guinea pig brains. The primate brains also revealed a different distribution binding profile for these various NPY receptor subtypes. Although the human and vervet brains contained very low amounts of [125I][Leu31,Pro34]PYY sites (Y1-like) in most brain regions, except for the dentate gyrus of the hippocampus, the marmoset brain contains significant amounts of both [125I][Leu31,Pro34]PYY (Y1-like) and [125I]PYY3-36 (Y2-like) binding sites. Additionally, [125I][Leu31,Pro34]PYY/BIBP3226-insensitive binding sites were not clearly detected in the vervet and human brains. On the other hand, Y5-like binding sites were observed in few regions of the marmoset brain. Finally, [125I]hPP (Y4/Y5-like) were very discretely distributed in the rat brain, being concentrated in the paraventricular nucleus of the hypothalamus and the interpeduncular nucleus. The marmoset brain is apparently not enriched with specific [125I]hPP sites. Taken together, these data show that significant species differences exist in the level of expression and distribution of various NPY receptor subtypes in the mammalian brain.
研究了代表三个哺乳动物目（啮齿动物（大鼠和小鼠）、豚鼠和灵长类动物（狨猴、黑长尾猴和人类））大脑中神经肽Y Y1、Y2、Y4和Y5受体亚型的各自分布情况。[125I][亮氨酸31，脯氨酸34]PYY（总Y1样；Y1、Y4和Y5）和[125I]PYY3 - 36（总Y2样；Y2以及可能的Y5）结合位点在大鼠和小鼠大脑中呈离散但相似的分布，各自具有独特的模式。相比之下令人惊讶的是，在豚鼠大脑包括海马结构中发现[125I]PYY3 - 36结合位点水平很低。在大鼠和豚鼠大脑的不同区域发现了[125I][亮氨酸31，脯氨酸34]PYY/BIBP3226不敏感结合位点（Y5样）。灵长类动物大脑对于这些不同的神经肽Y受体亚型也显示出不同的结合分布特征。虽然人类和黑长尾猴大脑在大多数脑区中[125I][亮氨酸31，脯氨酸34]PYY位点（Y1样）含量非常低，除了海马齿状回，但狨猴大脑中含有大量的[125I][亮氨酸31，脯氨酸34]PYY（Y1样）和[125I]PYY3 - 36（Y2样）结合位点。此外，在黑长尾猴和人类大脑中未清晰检测到[125I][亮氨酸31，脯氨酸34]PYY/BIBP3226不敏感结合位点。另一方面，在狨猴大脑的少数区域观察到Y5样结合位点。最后，[125I]人胰多肽（Y4/Y5样）在大鼠大脑中分布非常离散，集中在下丘脑室旁核和脚间核。狨猴大脑显然没有富含特定的[125I]人胰多肽位点。综上所述，这些数据表明在哺乳动物大脑中各种神经肽Y受体亚型的表达水平和分布存在显著的物种差异。
