Andersson M, Girard R, Cazenave P
Immunochimie Analytique, URA CNRS 1961, Departement d'Immunologie, Institut Pasteur, Paris, France.
Infect Immun. 1999 Jan;67(1):201-5. doi: 10.1128/IAI.67.1.201-205.1999.
NK lysin is a 9-kDa polypeptide that was originally isolated from porcine intestinal tissue based on its antibacterial activity. It is produced by cytolytic lymphocytes and is cytolytic against a number of different types of tumor cells. Here we report the binding of NK lysin to lipopolysaccharide (LPS) and its anti-LPS activity. NK lysin binds to matrix-coated LPS from Escherichia coli, Pseudomonas aeruginosa, and different strains of Salmonella enterica. Lipid A and polymyxin B inhibited the binding, demonstrating a preferential interaction of NK lysin with the lipid part of LPS. Chromium-labeled lymphoma cells were lysed by NK lysin, and LPS dose-dependently inhibited the cytolysis at equimolar amounts. In the same manner, NK lysin inhibited certain LPS-stimulated effects on mouse bone marrow cells as well as LPS binding to mouse granulocytes. These results suggest that NK lysin may be a another natural LPS-binding protein from lymphocytes that may participate in the endogenous defense response associated with elevated concentrations of LPS.
NK溶素是一种9千道尔顿的多肽，最初是基于其抗菌活性从猪肠道组织中分离出来的。它由溶细胞性淋巴细胞产生，对多种不同类型的肿瘤细胞具有溶细胞作用。在此我们报告NK溶素与脂多糖（LPS）的结合及其抗LPS活性。NK溶素可与来自大肠杆菌、铜绿假单胞菌和不同肠炎沙门氏菌菌株的基质包被LPS结合。脂多糖A和多粘菌素B可抑制这种结合，表明NK溶素与LPS的脂质部分存在优先相互作用。铬标记的淋巴瘤细胞可被NK溶素裂解，且LPS在等摩尔量时呈剂量依赖性抑制细胞裂解。同样，NK溶素也抑制LPS对小鼠骨髓细胞的某些刺激作用以及LPS与小鼠粒细胞的结合。这些结果表明，NK溶素可能是淋巴细胞产生的另一种天然LPS结合蛋白，可能参与与LPS浓度升高相关的内源性防御反应。
