Jain S D, Uppal B, Mehta D K
Department of Microbiology, Safdarjang Hospital.
Indian J Pathol Microbiol. 1998 Oct;41(4):387-90.
200 uveitis cases and 100 controls were serologically analysed for Toxoplasma antibodies (Ab) using indirect fluorescent antibody test (IFAT-IgG, Igm) and enzyme linked immunosorbent assay (ELISA IgG, IgM). Ophthalmologically cases were segregated into 4 groups anterior uveitis, posterior uveitis, pan uveitis and varied presentation uveitis. Toxoplasma seropositivity of 32% in cases and 4% in controls was established. IHA, IFAT, ELISA detected 20%, 18% and 32% cases as seropositive respectively, IFAT being most specific (100%) and ELISA most sensitive (41.37%). Insignificant change in Ab titre was observed in sequential samples of seropositive cases. Posterior Uveitis cases had the maximum seropositivity (41.7%). Highest seropositivity was in 16-25 years age group with no sex preponderance. Dietary habits and occupational history had no bearing on Toxoplasma infection. Results indicate that serology in mandatory for diagnosing Toxoplasma as a cause of uveitis, 2 or more tests on a single serum sample detecting IgG and Igm Ab are the best indicators of infection.
采用间接荧光抗体试验（IFAT-IgG、IgM）和酶联免疫吸附测定（ELISA IgG、IgM）对200例葡萄膜炎患者和100例对照进行弓形虫抗体血清学分析。眼科方面，病例分为4组：前葡萄膜炎、后葡萄膜炎、全葡萄膜炎和表现多样的葡萄膜炎。病例组弓形虫血清阳性率为32%，对照组为4%。间接血凝试验（IHA）、间接荧光抗体试验（IFAT）、酶联免疫吸附测定（ELISA）分别检测出20%、18%和32%的病例血清阳性，其中IFAT特异性最高（100%），ELISA敏感性最高（41.37%）。在血清阳性病例的连续样本中，未观察到抗体滴度有显著变化。后葡萄膜炎病例的血清阳性率最高（41.7%）。16至25岁年龄组血清阳性率最高，无性别优势。饮食习惯和职业史与弓形虫感染无关。结果表明，血清学检查对于诊断弓形虫引起的葡萄膜炎必不可少，对单个血清样本进行2次或更多次检测IgG和IgM抗体是感染的最佳指标。
