前列腺素A1和B2对Vero细胞中马亚罗病毒复制的抑制作用。

Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells.

作者信息

Ishimaru D, Marcicano F G, Rebello M A

机构信息

Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.

出版信息

Braz J Med Biol Res. 1998 Sep;31(9):1119-23. doi: 10.1590/s0100-879x1998000900003.

DOI:10.1590/s0100-879x1998000900003

PMID:9876277

Abstract

The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 micrograms/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.

摘要

在非洲绿猴肾细胞（Vero细胞）中研究了前列腺素（PGA1和PGB2）对马亚罗病毒复制的影响。发现PGA1和PGB2的抗病毒活性呈剂量依赖性。然而，虽然10微克/毫升的PGB2可使病毒产量降低60%，但在相同剂量下，PGA1可使病毒复制抑制超过90%。对[35S] - 甲硫氨酸标记蛋白的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析表明，PGA1不会改变细胞蛋白质合成。在受感染的细胞中，PGA1略微抑制蛋白C的合成，同时显著抑制糖蛋白E1和E2的合成。