肯尼亚莱基皮亚区利什曼原虫分离株的特征分析

Characterisation of Leishmania isolates from Laikipia District, Kenya.

作者信息

Massamba N N, Mutinga M J, Kamau C C

机构信息

The International Centre of Insect Physiology and Ecology, Nairobi, Kenya.

出版信息

Acta Trop. 1998 Nov 30;71(3):293-303. doi: 10.1016/s0001-706x(98)00071-0.

DOI:10.1016/s0001-706x(98)00071-0

PMID:9879738

Abstract

Using cloned kDNA sequences from the reference strain of Leishmania tropica IC-067, we have screened leishmanial isolates from Laikipia District of Kenya and identified these isolates as L. tropica. One isolate, IC-331, from a rat (Rattus rattus) was typed as L. tropica. This is the first time that L. tropica has been isolated from a wild rodent in Kenya and thus the first indication that the disease caused by L. tropica might be a zoonosis. The leishmanial isolates, especially those of sandflies, display extensive heterogeneity, prompting a need for selecting local and/or regional reference strains to generate species-specific DNA probes for use in identifying field isolates.

摘要

利用来自热带利什曼原虫IC - 067参考菌株的克隆kDNA序列，我们对肯尼亚莱基皮亚区的利什曼原虫分离株进行了筛选，并将这些分离株鉴定为热带利什曼原虫。从一只大鼠（黑家鼠）分离出的一个菌株IC - 331被鉴定为热带利什曼原虫。这是首次在肯尼亚从野生啮齿动物中分离出热带利什曼原虫，因此首次表明由热带利什曼原虫引起的疾病可能是人畜共患病。利什曼原虫分离株，尤其是白蛉的分离株，表现出广泛的异质性，这促使我们需要选择当地和/或区域参考菌株来生成用于鉴定野外分离株的物种特异性DNA探针。