通过聚合酶链反应对B亚属腺病毒（3型、7型和11型）进行选择性检测和鉴别

Selective detection and differentiation of subgenus B adenoviruses (types 3, 7 and 11) by polymerase chain reaction.

作者信息

Fujimoto T, Chikahira M, Nishio O

机构信息

Division of Microbiology, Hyogo Prefectural Institute of Public Health, Kobe, Japan.

出版信息

Kansenshogaku Zasshi. 1998 Nov;72(11):1202-7. doi: 10.11150/kansenshogakuzasshi1970.72.1202.

DOI:10.11150/kansenshogakuzasshi1970.72.1202

PMID:9884507

Abstract

Application of the polymerase chain reaction (PCR) method for detection of subgenus B adenoviruses (types 3, 7 and 11) was investigated. It is based on a simple (nonnested) PCR using primer pairs specific for the hexon-coding region. The PCR allowed amplification of DNA from subgenus B adenovirus prototype strains (types 3, 7 and 11) and adenovirus isolates (types 3 and 7), whereas it did not amplify DNA from subgenus A (type 31), C (types 1, 2, 5 and 6), D (types 8, 19 and 37), E (type 4) and adenovirus isolates (types 1, 2, 5 and 6). These results suggest that subgenus B adenoviruses (types 3, 7 and 11) are detectable selectively by means of PCR with primer pairs developed in this study. Amplified fragments from adenovirus types 3, 7 and 11 could be differentiated with restriction endonuclease analysis with Rsa I.

摘要

对聚合酶链反应（PCR）方法用于检测B亚属腺病毒（3型、7型和11型）进行了研究。该方法基于一种简单（非巢式）PCR，使用针对六邻体编码区的引物对。PCR能够扩增B亚属腺病毒原型株（3型、7型和11型）及腺病毒分离株（3型和7型）的DNA，而不能扩增A亚属（31型）、C亚属（1型、2型、5型和6型）、D亚属（8型、19型和37型）、E亚属（4型）的腺病毒及腺病毒分离株（1型、2型、5型和6型）的DNA。这些结果表明，利用本研究中开发的引物对通过PCR可选择性地检测B亚属腺病毒（3型、7型和11型）。来自3型、7型和11型腺病毒的扩增片段可用Rsa I进行限制性内切酶分析来区分。