Cellek S, Moncada S
The Wolfson Institute for Biomedical Research, University College London.
Br J Pharmacol. 1998 Dec;125(8):1627-9. doi: 10.1038/sj.bjp.0702278.
The corpus cavernosum is the erectile tissue in the penis and clitoris. Although nitrergic neurotransmission has been characterized in detail in the penile corpus cavernosum, functional studies on the inhibitory non-adrenergic non-cholinergic (NANC) transmission in the clitoral corpus cavernosum have been lacking. Here we demonstrate that electrical field stimulation (EFS) induces NANC relaxation responses in the clitoral corpus cavernosum of the rabbit. These responses were inhibited by NG-nitro-L-arginine methylester (L-NAME), 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ) or tetrodotoxin. The inhibitory effect of L-NAME was partially reversed by L-arginine but not by D-arginine. EFS-induced relaxations were enhanced by an inhibitor of type V cyclic GMP phosphodiesterase, zaprinast. These results suggest that nitrergic neurotransmission is responsible for the NANC relaxation responses in the clitoral corpus cavernosum of the rabbit.
海绵体是阴茎和阴蒂中的勃起组织。虽然对阴茎海绵体中的一氧化氮能神经传递已有详细描述,但对阴蒂海绵体中抑制性非肾上腺素能非胆碱能(NANC)传递的功能研究却一直缺乏。在此,我们证明电场刺激(EFS)可诱导兔阴蒂海绵体产生NANC舒张反应。这些反应可被NG-硝基-L-精氨酸甲酯(L-NAME)、1H-[1,2,4]恶二唑并[4,3,-a]喹喔啉-1-酮(ODQ)或河豚毒素抑制。L-NAME的抑制作用可被L-精氨酸部分逆转,但不能被D-精氨酸逆转。V型环磷酸鸟苷磷酸二酯酶抑制剂扎普司特可增强EFS诱导的舒张作用。这些结果表明,一氧化氮能神经传递介导了兔阴蒂海绵体的NANC舒张反应。