类固醇生成急性调节蛋白（StAR）和细胞色素P450c17在转化生长因子β1（TGFβ1）诱导的肾上腺皮质类固醇生成减少中的差异作用

Differential implication of StAR and P450c17 in TGFbeta1-induced decrease of adrenocortical steroidogenesis.

作者信息

Brand C, Bailly S, Defaye G, Chambaz E M, Feige J J

机构信息

INSERM Unit 244, Department of Molecular and Structural Biology, CEA/G, Grenoble, France.

出版信息

Endocr Res. 1998 Aug-Nov;24(3-4):763-8. doi: 10.3109/07435809809032684.

DOI:10.3109/07435809809032684

PMID:9888574

Abstract

In primary cultures of bovine adrenocortical fasciculata cells, we have previously identified two targets of TGFbeta action: StAR and P450c17. Since it is well known that adrenocortical cells tend to dedifferentiate as soon as they are placed in culture, we investigated the regulation of StAR and P450c17 expression by TGFbeta1 at different times of primary culture. On day 1, TGFbeta1 decreased the expression of basal levels of both genes. However, in the presence of ACTH, it down-regulated StAR mRNA levels but did not modify P450c17 mRNA levels. On day 4, P450c17 and StAR mRNAs were down-regulated by TGFbeta1, both in the absence and in the presence of ACTH. These observations indicate that StAR is likely to represent the major in vivo target of TGFbeta1 action in the adrenal cortex.

摘要

在牛肾上腺皮质束状带细胞的原代培养中，我们之前已确定转化生长因子β（TGFβ）作用的两个靶点：类固醇生成急性调节蛋白（StAR）和细胞色素P450c17。由于众所周知，肾上腺皮质细胞一旦置于培养中就容易去分化，我们研究了在原代培养的不同时间点，TGFβ1对StAR和P450c17表达的调控。在第1天，TGFβ1降低了这两个基因基础水平的表达。然而，在促肾上腺皮质激素（ACTH）存在的情况下，它下调了StAR mRNA水平，但未改变P450c17 mRNA水平。在第4天，无论有无ACTH，TGFβ1均下调了P450c17和StAR mRNA水平。这些观察结果表明，StAR可能是TGFβ1在肾上腺皮质中体内作用的主要靶点。