恶臭假单胞菌中xcp基因簇的分子组织：缺乏xcpX（gspK）同源物。
Molecular organization of the xcp gene cluster in Pseudomonas putida: absence of an xcpX (gspK) homologue.
作者信息
de Groot A, Gerritse G, Tommassen J, Lazdunski A, Filloux A
机构信息
Laboratoire d'Ingéniérie des Systèmes Macromoléculaires, UPR9027, IBSM/CNRS, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France.
出版信息
Gene. 1999 Jan 8;226(1):35-40. doi: 10.1016/s0378-1119(98)00570-8.
A DNA fragment containing xcp (gsp) gene homologues, required for extracellular protein secretion by the general secretory pathway (GSP) in various Gram-negative bacteria, was cloned from Pseudomonas putida (Pp) strain WCS358 and sequenced. The results presented here and those previously reported (de Groot, A., Krijger, J.-J., Filloux, A., Tommassen, J., 1996. Characterization of type II protein secretion (xcp) genes in the plant growth-stimulating Pseudomonas putida, strain WCS358 Mol. Gen. Genet. 250, 491-504) complete the sequence of the xcp gene cluster of Pp. Unlike that of Pseudomonas aeruginosa (Pa), the xcp gene cluster of Pp contains a gspN homologue. More surprisingly, in contrast to all known gsp gene clusters, the xcpX (gspK) homologue is not found. In addition, genes flanking the xcp cluster of Pp are not related to those flanking the xcp genes of Pa. Overall, the xcp gene products of Pp are as much related to those of Pa as to gsp gene products of enterobacterial species, suggesting that the xcp clusters of Pp and Pa have evolved separately.
从恶臭假单胞菌（Pp）菌株WCS358中克隆并测序了一段DNA片段，该片段含有xcp（gsp）基因同源物，它是多种革兰氏阴性菌通过一般分泌途径（GSP）进行细胞外蛋白分泌所必需的。本文给出的结果以及先前报道的结果（de Groot, A., Krijger, J.-J., Filloux, A., Tommassen, J., 1996. Characterization of type II protein secretion (xcp) genes in the plant growth-stimulating Pseudomonas putida, strain WCS358 Mol. Gen. Genet. 250, 491-504）完善了Pp的xcp基因簇序列。与铜绿假单胞菌（Pa）不同，Pp的xcp基因簇含有一个gspN同源物。更令人惊讶的是，与所有已知的gsp基因簇相比，未发现xcpX（gspK）同源物。此外，Pp的xcp簇两侧的基因与Pa的xcp基因两侧的基因无关。总体而言, Pp的xcp基因产物与Pa的xcp基因产物的相关性与和肠杆菌科物种的gsp基因产物的相关性一样高，这表明Pp和Pa的xcp簇是独立进化的。
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