McNicol P J, Paraskevas M, Guijon F B
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Cancer Detect Prev. 1999;23(1):13-21. doi: 10.1046/j.1525-1500.1999.00069.x.
Since other viruses can influence expression of human papillomavirus (HPV) 16 E6-E7 genes in vitro, this study addressed whether specific vaginal bacteria do so as well. The vaginal microflora of 18 women with cervical intraepithelial neoplasia (CIN) or normal histology and HPV 16 infection, was evaluated by quantitative culture. Expression of HPV 16 E6-E7 oncogenes was assessed in exfoliated cervical cells by quantitative polymerase chain reaction. HPV 16 expression was also quantitated in CaSki carcinoma cell line cocultured with Bacteroides fragilis or Lactobacillus acidophilus. Isolation of Lactobacillus sp. (p = 0.05) and expression of the E6*II transcript (p = 0.03) were associated with low-grade CIN or normal histology. However, changes in E6-E7 expression were not associated independently with isolation of a specific microorganism. Similarly, expression of HPV 16 E6-E7 oncogenes in vitro was unaltered in the presence of bacteria. These results suggest that vaginal microorganisms are unlikely to alter the natural history of HPV-associated CIN by influencing HPV oncogene expression.
由于其他病毒可在体外影响人乳头瘤病毒（HPV）16 E6 - E7基因的表达，本研究探讨特定阴道细菌是否也有此作用。通过定量培养评估了18名患有宫颈上皮内瘤变（CIN）或组织学正常且感染HPV 16的女性的阴道微生物群。通过定量聚合酶链反应评估宫颈脱落细胞中HPV 16 E6 - E7癌基因的表达。还对与脆弱拟杆菌或嗜酸乳杆菌共培养的CaSki癌细胞系中的HPV 16表达进行了定量。乳酸杆菌属的分离（p = 0.05）和E6*II转录本的表达（p = 0.03）与低级别CIN或组织学正常相关。然而，E6 - E7表达的变化与特定微生物的分离并无独立相关性。同样，体外HPV 16 E6 - E7癌基因的表达在有细菌存在时未发生改变。这些结果表明，阴道微生物不太可能通过影响HPV癌基因表达来改变HPV相关CIN的自然病程。
