色氨酸热解产物Trp-P-1和Trp-P-2可诱导原代培养的大鼠肝细胞凋亡。

Tryptophan pyrolysis products, Trp-P-1 and Trp-P-2 induce apoptosis in primary cultured rat hepatocytes.

作者信息

Ashida H, Shiotani B, Adachi H, Hashimoto T, Kanazawa K, Danno G

机构信息

Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan.

出版信息

Biosci Biotechnol Biochem. 1998 Nov;62(11):2283-7. doi: 10.1271/bbb.62.2283.

DOI:10.1271/bbb.62.2283

PMID:9972255

Abstract

The cytotoxicity of heterocyclic amines, dietary carcinogens derived from cooked foods, to primary cultured rat hepatocytes was studied. A tryptophan pyrolysis product, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) was the most cytotoxic of 11 compounds tested. Trp-P-1 was found to induce apoptosis as measured by morphological changes in nuclear chromatin and internucleosomal DNA fragmentation. 3-Amino-1-methyl-5H-pyrido[4,3-b] indole (Trp-P-2) showed a moderate apoptotic effect, and other compounds had a similar but weaker effect.

摘要

研究了杂环胺（一种源自熟食的膳食致癌物）对原代培养大鼠肝细胞的细胞毒性。色氨酸热解产物3-氨基-1,4-二甲基-5H-吡啶并[4,3-b]吲哚（Trp-P-1）是所测试的11种化合物中细胞毒性最强的。通过核染色质的形态变化和核小体间DNA片段化测量发现，Trp-P-1可诱导细胞凋亡。3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚（Trp-P-2）显示出中等程度的凋亡作用，其他化合物也有类似但较弱的作用。