紫花苜蓿含有大量的9-氢过氧化物裂解酶活性和一种3Z:2E-烯醛异构酶。

Alfalfa contains substantial 9-hydroperoxide lyase activity and a 3Z:2E-enal isomerase.

作者信息

Noordermeer M A, Veldink G A, Vliegenthart J F

机构信息

Bijvoet Center for Biomolecular Research, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.

出版信息

FEBS Lett. 1999 Jan 25;443(2):201-4. doi: 10.1016/s0014-5793(98)01706-2.

DOI:10.1016/s0014-5793(98)01706-2

PMID:9989605

Abstract

Fatty acid hydroperoxides formed by lipoxygenase can be cleaved by hydroperoxide lyase resulting in the formation of short-chain aldehydes and omega-oxo acids. Plant hydroperoxide lyases use 13- or 9-hydroperoxy linoleic and linolenic acid as substrates. Alfalfa (Medicago sativa L.) has been reported to contain a hydroperoxide lyase specific for 13-hydroperoxy linoleic and linolenic acid only. However, in addition to 13-hydroperoxide lyase activity we found substantial 9-hydroperoxide lyase activity in alfalfa seedlings as well. The specific activity for 9-hydroperoxy fatty acids was about 50% of the activity for the 13-isomers. Furthermore, alfalfa seedlings contain a 3Z:2E-enal isomerase that converts the 3Z-enal products to their 2E-enal isoforms.

摘要

脂氧合酶形成的脂肪酸氢过氧化物可被氢过氧化物裂解酶裂解，从而形成短链醛和ω-氧代酸。植物氢过氧化物裂解酶以13-或9-氢过氧亚油酸和亚麻酸为底物。据报道，苜蓿（紫花苜蓿）仅含有一种对13-氢过氧亚油酸和亚麻酸具有特异性的氢过氧化物裂解酶。然而，除了13-氢过氧化物裂解酶活性外，我们还在苜蓿幼苗中发现了大量的9-氢过氧化物裂解酶活性。9-氢过氧脂肪酸的比活性约为13-异构体活性的50%。此外，苜蓿幼苗含有一种3Z:2E-烯醛异构酶，可将3Z-烯醛产物转化为其2E-烯醛异构体。