Ruhnke H L, Thawley D, Nelson F C
Can J Comp Med. 1976 Apr;40(2):142-8.
Bovine mastitis caused by Mycoplasma agalactiae subsp. bovis was first diagnosed in 16 of 55 cows in an Ontario herd in Feburary 1972. A total of 182 of 598 (30.4%) cows from 33 of 64 (51.5%) farms in widely separated areas of the province were culturally positive. Herd incidence varied from 15 to 40% with one closed herd having an incidence of 61%. Four herds were investigated culturally and serologically by the growth inhibition test for 15 months. In the acute phase the organism was present in the milk in extremely high numbers and could still be isolated from a few cows after eight to 12 months. The sera from 89.5% of the animals with clinical mycoplasma mastitis produced a zone of surface "film" and/or colony inhibition and some cows remained positive for six to 12 months. The disease was experimentally reproduced with a pure culture of the organism isolated from the milk of a cow from one of the herds.
1972年2月,安大略省一群奶牛中的55头奶牛中有16头首次被诊断出由无乳支原体牛亚种引起的牛乳腺炎。该省广泛分布地区的64个农场中的33个农场的598头奶牛(30.4%)中,共有182头奶牛培养呈阳性。牛群发病率从15%到40%不等,其中一个封闭牛群的发病率为61%。对四个牛群进行了为期15个月的培养和血清学生长抑制试验调查。在急性期,牛奶中存在大量该病原体,8至12个月后仍能从少数奶牛中分离出来。89.5%患有临床支原体乳腺炎的动物血清产生表面“薄膜”区和/或菌落抑制,一些奶牛在6至12个月内仍呈阳性。用从其中一个牛群的奶牛乳汁中分离出的该病原体的纯培养物,通过实验再现了这种疾病。