Schelbert H R, Ingwall J S, Sybers H D, Ashburn W L
Circ Res. 1976 Dec;39(6):860-8. doi: 10.1161/01.res.39.6.860.
We studied the specificity of uptake of infarct-imaging agents for reversibly or irreversibly injured myocardium independently of blood flow by using intact beating fetal mouse hearts in organ culture. Reversible injury resulted from deprivation of oxygen and glucose for 4 hours at 37 degrees C; irreversible injury, from similar deprivation at 42 degrees C. At the end of the insult, uptake of 99mTc(Sc)-labeled pyrophosphate, glucoheptonate, or tetracycline was markedly increased in irreversibly damaged and, to a lesser degree, in reversibly injured hearts. After 24 hours of recovery, necrotic hearts accumulated even more pyrophosphate and tatracycline but less glucoheptonate. Uptake of radioiodinated tetracycline increased only in irreversibly injured hearts. Pyrophosphate uptake was not reduced in hearts cultured in calcium-free medium. These finding suggest that 99mTc(Sn)-labeled pyrophosphate, tetracycline, and glucoheptonate preferentially localize in irreversibly damaged myocardium; the 99mTc(Sn) complex modifies the specificity of uptake; and the uptake of 99mTc(Sn)-pyrophosphate appears unrelated to calcium uptake.
我们通过在器官培养中使用完整跳动的胎鼠心脏，独立于血流研究了梗死显像剂对可逆性或不可逆性损伤心肌的摄取特异性。可逆性损伤是在37℃下缺氧和缺葡萄糖4小时导致的；不可逆性损伤是在42℃下类似的剥夺导致的。在损伤结束时，99mTc(Sc)标记的焦磷酸盐、葡庚糖酸盐或四环素在不可逆损伤的心脏中摄取明显增加，在可逆性损伤的心脏中摄取增加程度较小。恢复24小时后，坏死心脏积累了更多的焦磷酸盐和四环素，但葡庚糖酸盐较少。放射性碘化四环素的摄取仅在不可逆损伤的心脏中增加。在无钙培养基中培养的心脏中，焦磷酸盐摄取并未减少。这些发现表明，99mTc(Sn)标记的焦磷酸盐、四环素和葡庚糖酸盐优先定位于不可逆损伤的心肌；99mTc(Sn)复合物改变了摄取特异性；并且99mTc(Sn)-焦磷酸盐的摄取似乎与钙摄取无关。
