Grimes J E, Page L A
Avian Dis. 1978 Jul-Sep;22(3):422-30.
Detection of chlamydial antibody in serums of wild birds was compared for the following methods: direct complement-fixation (DCF), modified DCF (MDCF), and agar-gel precipitin (AGP). The birds species used were great-tailed grackles (Cassidix mexicanus), common grackles (Quiscalus quiscula), brown-headed cowbirds (Molothrus ater), bronzed cowbirds (Tangavius aeneus), and mourning doves (Zenaida macroura). The birds were either inoculated with an isolate of Chlamydia psittaci obtained originally from turkeys or kept as uninoculated cagemates. DCF detected antibody in most inoculated common grackles, mourning doves, and brown-headed cowbirds. It was unreliable, however, for great-tailed grackles and bronzed cowbirds inoculated intramuscularly. MDCF method detected antibody in all inoculated birds except 1 mourning dove, and gave higher titers than did DCF. AGP detected antibody in all inoculated brown-headed cowbirds and all mourning doves, 1 inoculated and 1 exposed great-tailed grackle, and none of the other 2 species. Chlamydiae were apparently transmitted to the uninoculated great-tailed and common grackles and mourning doves, for antibody was detected by all 3 methods in these species kept as uninoculated cagemates.
直接补体结合试验（DCF）、改良DCF试验（MDCF）和琼脂凝胶沉淀试验（AGP）。所用鸟类物种为长尾拟八哥（Cassidix mexicanus）、紫翅椋鸟（Quiscalus quiscula）、褐头牛鹂（Molothrus ater）、铜色牛鹂（Tangavius aeneus）和哀鸽（Zenaida macroura）。这些鸟类要么接种最初从火鸡分离得到的鹦鹉热衣原体菌株，要么作为未接种的同笼鸟饲养。DCF在大多数接种的紫翅椋鸟、哀鸽和褐头牛鹂中检测到抗体。然而，对于肌肉注射接种的长尾拟八哥和铜色牛鹂，该方法不可靠。MDCF方法在除1只哀鸽外的所有接种鸟类中检测到抗体，且滴度高于DCF。AGP在所有接种的褐头牛鹂和所有哀鸽、1只接种和1只接触过的长尾拟八哥中检测到抗体，在其他2个物种中均未检测到。衣原体显然传播到了未接种的长尾拟八哥、紫翅椋鸟和哀鸽，因为在作为未接种同笼鸟饲养的这些物种中，所有3种方法均检测到了抗体。
