光合细菌红螺菌中二磷酸核酮糖羧化酶的动力学研究。
A kinetic study of ribulose bisphosphate carboxylase from the photosynthetic bacterium Rhodospirillum rubrum.
作者信息
Christeller J T, Laing W A
出版信息
Biochem J. 1978 Aug 1;173(2):467-73. doi: 10.1042/bj1730467.
Abstract
The activation kinetics of purified Rhodospirillum rubrum ribulose bisphosphate carboxylase were analysed. The equilibrium constant for activation by CO(2) was 600 micron and that for activation by Mg2+ was 90 micron, and the second-order activation constant for the reaction of CO(2) with inactive enzyme (k+1) was 0.25 X 10(-3)min-1 . micron-1. The latter value was considerably lower than the k+1 for higher-plant enzyme (7 X 10(-3)-10 X 10(-3)min-1 . micron-1). 6-Phosphogluconate had little effect on the active enzyme, and increased the extent of activation of inactive enzyme. Ribulose bisphosphate also increased the extent of activation and did not inhibit the rate of activation. This effect might have been mediated through a reaction product, 2-phosphoglycolic acid, which also stimulated the extent of activation of the enzyme. The active enzyme had a Km (CO2) of 300 micron-CO2, a Km (ribulose bisphosphate) of 11--18 micron-ribulose bisphosphate and a Vmax. of up to 3 mumol/min per mg of protein. These data are discussed in relation to the proposed model for activation and catalysis of ribulose bisphosphate carboxylase.
摘要
对纯化的深红红螺菌核酮糖二磷酸羧化酶的激活动力学进行了分析。CO₂激活的平衡常数为600微摩尔，Mg²⁺激活的平衡常数为90微摩尔，CO₂与无活性酶反应的二级激活常数（k⁺¹）为0.25×10⁻³分钟⁻¹·微摩尔⁻¹。后一数值明显低于高等植物酶的k⁺¹（7×10⁻³ - 10×10⁻³分钟⁻¹·微摩尔⁻¹）。6 - 磷酸葡萄糖酸对活性酶影响很小，但增加了无活性酶的激活程度。核酮糖二磷酸也增加了激活程度且不抑制激活速率。这种效应可能是通过反应产物2 - 磷酸乙醇酸介导的，2 - 磷酸乙醇酸也刺激了该酶的激活程度。活性酶的Km（CO₂）为300微摩尔CO₂，Km（核酮糖二磷酸）为11 - 18微摩尔核酮糖二磷酸，Vmax高达每毫克蛋白质3微摩尔/分钟。结合所提出的核酮糖二磷酸羧化酶激活和催化模型对这些数据进行了讨论。
相似文献
1
A kinetic study of ribulose bisphosphate carboxylase from the photosynthetic bacterium Rhodospirillum rubrum.光合细菌红螺菌中二磷酸核酮糖羧化酶的动力学研究。
Biochem J. 1978 Aug 1;173(2):467-73. doi: 10.1042/bj1730467.
2
Ribulose bisphosphate carboxylase/oxygenase in toluene-permeabilized Rhodospirillum rubrum.甲苯通透的红螺菌中的1,5-二磷酸核酮糖羧化酶/加氧酶
Biochem J. 1983 Apr 15;212(1):45-54. doi: 10.1042/bj2120045.
3
Activation and regulation of ribulose bisphosphate carboxylase-oxygenase in the absence of small subunits.在缺乏小亚基的情况下核酮糖二磷酸羧化酶-加氧酶的激活与调控
J Biol Chem. 1979 Oct 25;254(20):10184-9.
4
Inhibition of ribulose bisphosphate carboxylase by substrate ribulose 1,5-bisphosphate.底物1,5-二磷酸核酮糖对二磷酸核酮糖羧化酶的抑制作用。
J Biol Chem. 1983 Nov 25;258(22):13752-8.
5
Role of the large and small subunits of ribulose-1,5-bisphosphate carboxylase in the activation by CO2 and Mg2+.1,5-二磷酸核酮糖羧化酶的大亚基和小亚基在二氧化碳和镁离子激活过程中的作用。
J Biochem. 1979 Apr;85(4):923-30. doi: 10.1093/oxfordjournals.jbchem.a132424.
6
A model for the kinetics of activation and catalysis of ribulose 1,5-bisphosphate carboxylase.1,5-二磷酸核酮糖羧化酶激活与催化动力学模型
Biochem J. 1976 Dec 1;159(3):563-70. doi: 10.1042/bj1590563.
7
The effects of bivalent cations on ribulose bisphosphate carboxylase/oxygenase.二价阳离子对核酮糖二磷酸羧化酶/加氧酶的影响。
Biochem J. 1981 Mar 1;193(3):839-44. doi: 10.1042/bj1930839.
8
Modification of ribulose bisphosphate carboxylase from Rhodospirillum rubrum with tetranitromethane.用四硝基甲烷对深红红螺菌的二磷酸核酮糖羧化酶进行修饰。
Biochem Biophys Res Commun. 1979 May 14;88(1):85-91. doi: 10.1016/0006-291x(79)91699-1.
9
Differential effects of metal ions on Rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase and stoichiometric incorporation of HCO3- into a cobalt(III)--enzyme complex.金属离子对深红红螺菌核酮糖二磷酸羧化酶/加氧酶的不同影响以及HCO₃⁻化学计量地掺入钴（III）-酶复合物中
Biochemistry. 1979 Oct 16;18(21):4453-8. doi: 10.1021/bi00588a001.
10
Carbon isotope effect on carboxylation of ribulose bisphosphate catalyzed by ribulosebisphosphate carboxylase from Rhodospirillum rubrum.碳同位素对红螺菌中核酮糖二磷酸羧化酶催化的核酮糖二磷酸羧化作用的影响。
Biochemistry. 1985 Mar 26;24(7):1603-7. doi: 10.1021/bi00328a005.
引用本文的文献
1
The response to ribulose bisphosphate(4-) (RuBP (4-)) and RuBP-Mg (2-) in catalysis by structurally divergent RuBP carboxylase/oxygenases.结构不同的核酮糖二磷酸羧化酶/加氧酶对核酮糖二磷酸（4-）（RuBP（4-））和 RuBP-Mg（2-）在催化反应中的响应。
Photosynth Res. 1983 Jun;4(2):111-7. doi: 10.1007/BF00052372.
2
The response to ribulose bisphosphate(4-) (RuBP (4-)) and RuBP-Mg (2-) in catalysis by structurally divergent RuBP carboxylase/oxygenases.结构不同的核酮糖二磷酸羧化酶/加氧酶在催化反应中对核酮糖二磷酸(4-)（RuBP (4-)）和 RuBP-Mg (2-)的响应。
Photosynth Res. 1983 Jan;4(1):111-7. doi: 10.1007/BF00041806.
3
Analysis of Chromophytic and Rhodophytic Ribulose-1,5-Bisphosphate Carboxylase Indicates Extensive Structural and Functional Similarities among Evolutionarily Diverse Algae.分析有色体和红色体的核酮糖-1,5-二磷酸羧化酶表明，在进化上不同的藻类之间存在广泛的结构和功能相似性。
Plant Physiol. 1989 Nov;91(3):939-46. doi: 10.1104/pp.91.3.939.
4
Kinetic Variance of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Isolated from Diverse Taxonomic Sources : II. Analysis by Two Dual Label Methods.来自不同分类来源的核酮糖-1,5-二磷酸羧化酶/加氧酶的动力学变异性：II. 两种双重标记方法的分析。
Plant Physiol. 1984 Jul;75(3):645-50. doi: 10.1104/pp.75.3.645.
5
Variations in K(m)(CO(2)) of Ribulose-1,5-bisphosphate Carboxylase among Grasses.禾本科植物中1,5-二磷酸核酮糖羧化酶的二氧化碳米氏常数（K(m)(CO(2))）的变化
Plant Physiol. 1980 Dec;66(6):1110-2. doi: 10.1104/pp.66.6.1110.
6
C nuclear magnetic resonance study of the CO(2) activation of ribulosebisphosphate carboxylase from Rhodospirillum rubrum.红螺菌中核酮糖二磷酸羧化酶二氧化碳活化的C核磁共振研究。
Proc Natl Acad Sci U S A. 1979 Feb;76(2):673-5. doi: 10.1073/pnas.76.2.673.
7
A site-specific mutation within the active site of ribulose-1,5-bisphosphate carboxylase of Rhodospirillum rubrum.红假单胞菌核酮糖-1,5-二磷酸羧化酶活性部位的一个特定位置突变。
EMBO J. 1984 Dec 1;3(12):2737-43. doi: 10.1002/j.1460-2075.1984.tb02204.x.
8
Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) supports photosynthesis and growth in tobacco.质体基因组编码的细菌核酮糖-1,5-二磷酸羧化酶/加氧酶（RubisCO）支持烟草的光合作用和生长。
Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14738-43. doi: 10.1073/pnas.261417298. Epub 2001 Nov 27.
9
Presence of two subunit types in ribulose 1,5-bisphosphate carboxylase from Thiobacillus intermedius.中间硫杆菌中1,5-二磷酸核酮糖羧化酶两种亚基类型的存在。
J Bacteriol. 1980 Feb;141(2):652-7. doi: 10.1128/jb.141.2.652-657.1980.
10
The effects of bivalent cations on ribulose bisphosphate carboxylase/oxygenase.二价阳离子对核酮糖二磷酸羧化酶/加氧酶的影响。
Biochem J. 1981 Mar 1;193(3):839-44. doi: 10.1042/bj1930839.
本文引用的文献
1
Isotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase: No Effect of Temperature or HCO(3) Concentration.1,5-二磷酸核酮糖羧化酶对同位素的区分：不受温度或碳酸氢根浓度的影响。
Plant Physiol. 1976 Apr;57(4):580-2. doi: 10.1104/pp.57.4.580.
2
Regulation of ribulose 1,5-diphosphate carboxylase by substrates and other metabolites: further evidence for several types of binding sites.1,5-二磷酸核酮糖羧化酶受底物及其他代谢物的调控：关于多种结合位点的进一步证据
Plant Physiol. 1975 Apr;55(4):720-6. doi: 10.1104/pp.55.4.720.
3
Activation of ribulose 1,5-diphosphate carboxylase by nicotinamide adenine dinucleotide phosphate and other chloroplast metabolites.核酮糖 1,5-二磷酸羧化酶的激活物是烟酰胺腺嘌呤二核苷酸磷酸和其他叶绿体代谢物。
Plant Physiol. 1974 Oct;54(4):556-9. doi: 10.1104/pp.54.4.556.
4
Activation and inhibition of ribulose 1,5-diphosphate carboxylase by 6-phosphogluconate.6-磷酸葡萄糖酸对1,5-二磷酸核酮糖羧化酶的激活与抑制作用
Plant Physiol. 1973 Oct;52(4):373-9. doi: 10.1104/pp.52.4.373.
5
Free energy changes and metabolic regulation in steady-state photosynthetic carbon reduction.稳态光合碳还原中的自由能变化与代谢调节
Biochim Biophys Acta. 1969 Oct 21;189(2):207-21. doi: 10.1016/0005-2728(69)90048-6.
6
Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.通过在SDS-聚丙烯酰胺凝胶上进行电泳来测定分子量。
Methods Enzymol. 1972;26:3-27. doi: 10.1016/s0076-6879(72)26003-7.
7
Regulation of ribulose-1,5-diphosphate carboxylase by 6-phospho-D-gluconate.6-磷酸-D-葡萄糖酸对1,5-二磷酸核酮糖羧化酶的调节作用
Biochem Biophys Res Commun. 1972 Sep 5;48(5):1153-9. doi: 10.1016/0006-291x(72)90831-5.
8
Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase.自养型二氧化碳同化作用与核酮糖二磷酸羧化酶的进化
Bacteriol Rev. 1973 Sep;37(3):289-319. doi: 10.1128/br.37.3.289-319.1973.
9
Phosphoglycolate production catalyzed by ribulose diphosphate carboxylase.由核酮糖二磷酸羧化酶催化的磷酸乙醇酸生成。
Biochem Biophys Res Commun. 1971 Nov 5;45(3):716-22. doi: 10.1016/0006-291x(71)90475-x.
Zotero 插件