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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Nordby G, Berg T, Nilsson M, Norum K R

Biochim Biophys Acta. 1976 Oct 21;450(1):69-77. doi: 10.1016/0005-2760(76)90299-x.

Lecithin:cholesterol acyltransferase is secreted from isolated rat heptocytes. 2. The secretion is stimulated when serum is added to the incubation medium. 3. Optimal conditions for secretion are: 5-10(6) hepatocytes per ml, 5 h incubation, pH 7.3-7.4 and 25% serum in the incubation medium. 4. Concomitantly with the secretion of lecithin:cholesterol acyltransferase there is a secretion of unesterified cholesterol and triacylglycerol. 5. Colchicine or cycloheximide in the incubation medium inhibits secretion of lecithin:cholesterol acyltransferase.

卵磷脂胆固醇酰基转移酶由分离出的大鼠肝细胞分泌。2. 向孵育培养基中添加血清时，分泌会受到刺激。3. 分泌的最佳条件为：每毫升5 - 10(6)个肝细胞、孵育5小时、pH值7.3 - 7.4以及孵育培养基中含25%血清。4. 伴随着卵磷脂胆固醇酰基转移酶的分泌，未酯化胆固醇和三酰甘油也会分泌。5. 孵育培养基中的秋水仙碱或放线菌酮会抑制卵磷脂胆固醇酰基转移酶的分泌。

Nordby G, Berg T, Nilsson M, Norum K R

Biochim Biophys Acta. 1976 Oct 21;450(1):69-77. doi: 10.1016/0005-2760(76)90299-x.

Lecithin:cholesterol acyltransferase is secreted from isolated rat heptocytes. 2. The secretion is stimulated when serum is added to the incubation medium. 3. Optimal conditions for secretion are: 5-10(6) hepatocytes per ml, 5 h incubation, pH 7.3-7.4 and 25% serum in the incubation medium. 4. Concomitantly with the secretion of lecithin:cholesterol acyltransferase there is a secretion of unesterified cholesterol and triacylglycerol. 5. Colchicine or cycloheximide in the incubation medium inhibits secretion of lecithin:cholesterol acyltransferase.

卵磷脂胆固醇酰基转移酶由分离出的大鼠肝细胞分泌。2. 向孵育培养基中添加血清时，分泌会受到刺激。3. 分泌的最佳条件为：每毫升5 - 10(6)个肝细胞、孵育5小时、pH值7.3 - 7.4以及孵育培养基中含25%血清。4. 伴随着卵磷脂胆固醇酰基转移酶的分泌，未酯化胆固醇和三酰甘油也会分泌。5. 孵育培养基中的秋水仙碱或放线菌酮会抑制卵磷脂胆固醇酰基转移酶的分泌。