Akhamanov S A, Borisov A Iu, Danelius R V, Kozlovskiĭ V S, Piskarskas A S
Biofizika. 1978 Sep-Oct;23(5):912-3.
Absorption changes of reaction centers from Rhodospirillum rubrum at 748, 796 and 870 nm induced by 532 and 870 nm picosecond light pulses were investigated with a picosecond spectrometer. Kinetics of absorption changes at 748 and 796 had an additional bleaching when induced by the 532 nm pulse, in comparison with those at 870 nm. The additional bleaching was interpreted as a result of the excitation energy transfer via spectral forms of pigments of reaction centers. The experimental results fit the mathematical simulation of the additional bleaching for the following set of rate constant values: intrinsic conversion to the lowest excited singlet state in bacteriopheophytine molecule--10(13) s-1, energy transfer from bacteriopheophytine to P800 3.10(12) s-1, from P800 to P870--2.10(12) s-1.
利用皮秒光谱仪研究了532和870纳米皮秒光脉冲诱导的红螺菌反应中心在748、796和870纳米处的吸收变化。与870纳米处相比，由532纳米脉冲诱导时，748和796纳米处吸收变化的动力学有额外的漂白现象。额外的漂白被解释为反应中心色素光谱形式的激发能量转移的结果。对于以下一组速率常数：细菌叶绿素分子内转化为最低激发单重态——10(13) 秒-1，从细菌叶绿素到P800的能量转移——3.10(12) 秒-1，从P800到P870——2.10(12) 秒-1，实验结果与额外漂白的数学模拟相符。
