An immunological determination of specific activities of enzymes: its use in quantification of cross reactivity between enzymes of different origins.
作者信息
Guiso N, Ullmann A
出版信息
Biochimie. 1978 Sep 4;60(5):467-71. doi: 10.1016/s0300-9084(78)80861-x.
PMID:100146
Abstract
An immunological method is presented which enables the determination of the specific activity of a pure enzyme without its extensive purification. The method consists essentially in the specific fixation of immunologically related enzymes to an immunoadsorbent containing specific antibodies raised against the wild-type form of the enzyme. We applied this method to determine the specific activity of plasmid-coded beta-galactosidases and to quantify the extent of cross-reaction between these enzymes and Escherichia coli beta-galactosidase.
摘要
相似文献
1
An immunological determination of specific activities of enzymes: its use in quantification of cross reactivity between enzymes of different origins.
Biochimie. 1978 Sep 4;60(5):467-71. doi: 10.1016/s0300-9084(78)80861-x.
2
Immunological relatedness between beta-galactosidases of different bacterial origins.不同细菌来源的β-半乳糖苷酶之间的免疫相关性。
Mol Immunol. 1979 Jun;16(6):409-11. doi: 10.1016/0161-5890(79)90108-1.
3
Efficient conjugation of rabbit Fab' with beta-D-galactosidase from Escherichia coli.兔Fab'与大肠杆菌β-D-半乳糖苷酶的高效偶联。
Scand J Immunol. 1979;10(1):81-6. doi: 10.1111/j.1365-3083.1979.tb01338.x.
4
5
An enzymatically active antigen-antibody probe to measure circulating immune complexes by competition. I. Use of Escherichia coli beta-galactosidase in the probe and of bovine conglutinin as the complex-binding reagent.一种通过竞争来检测循环免疫复合物的酶活性抗原-抗体探针。I. 探针中大肠杆菌β-半乳糖苷酶的应用以及牛胶固素作为复合物结合试剂的应用。
Clin Immunol Immunopathol. 1980 Jun;16(2):131-41. doi: 10.1016/0090-1229(80)90197-x.
6
Antibody-mediated activation of genetically defective Escherichia coli beta-galactosidases by monoclonal antibodies produced by somatic cell hybrids.通过体细胞杂交产生的单克隆抗体对基因缺陷型大肠杆菌β-半乳糖苷酶的抗体介导激活作用。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2478-82. doi: 10.1073/pnas.78.4.2478.
7
Relationships among raffinose plasmids determined by the immunochemical cross-reaction of their alpha-galactosidases.通过棉子糖质粒的α-半乳糖苷酶免疫化学交叉反应确定的棉子糖质粒之间的关系。
J Gen Microbiol. 1979 Oct;114(2):477-81. doi: 10.1099/00221287-114-2-477.
8
9
A comparison of the ability of beta-galactosidase and horseradish peroxidase enzyme-antibody conjugates to detect specific antibodies.
J Immunol Methods. 1979;31(3-4):247-50. doi: 10.1016/0022-1759(79)90137-6.
10
The localisation of phoE and beta-galactosidase antigens in wild type and phoE/1acZ hybrid Escherichia coli.
Cell Biol Int Rep. 1984 Mar;8(3):187. doi: 10.1016/0309-1651(84)90026-2.
Zotero 插件