Kriviskiĭ A S, Esipova V V, Tikhomirova L P, Koudelka Ia
Genetika. 1976;12(7):109-18.
Both acridine-sensitized inactivation and mutagenesis in phage sd have been studied and compared with the effect of other mutagens. Inactivation curve was not stictly exponential, with a small shoulder at short light doses and deviation of survival lower than 10(-5). The lethal effect was not reactivated by multiplicity reactivation. Photodynamic damage in phage sd was accompanied by the increase of the rise (but not of the latent) period in the one-step curve of phage multiplication and increase of the burst size. Experiments were carried out at dye concentration of 1-10(-5) M or lower; a strong dark effect of the dye being observed under 2-fold increase of the dye concentration. Plaque-type mutants were formed up to the maximum approximately 1% at the survival approximately 2--10(-5); in comparison with other mutagens photo-sensitized mutagenesis in phage sd was lower. The significant increase in the number of plaque mutants was observed after the illumination of phage fraction surviving the pre-treatment with higher acridine orange concentration (greater than or equal to 2-10(-5)M).
对噬菌体sd中吖啶敏化失活和诱变进行了研究，并与其他诱变剂的作用进行了比较。失活曲线并非严格呈指数形式，在短光照剂量下有一个小的肩部，且存活低于10（-5）时出现偏差。致死效应不能通过多重复活而复活。噬菌体sd中的光动力损伤伴随着噬菌体增殖一步曲线中上升期（而非潜伏期）的增加以及爆发量的增加。实验在染料浓度为1 - 10（-5）M或更低的情况下进行；在染料浓度增加2倍时观察到染料有很强的暗效应。在存活率约为2 - 10（-5）时，噬菌斑型突变体形成率最高约为1%；与其他诱变剂相比，噬菌体sd中的光致敏诱变作用较低。在用较高吖啶橙浓度（大于或等于2 - 10（-5）M）预处理后存活的噬菌体部分经光照后，观察到噬菌斑突变体数量显著增加。
