Wenk M, Von Hahn H P, Honegger C G
Hoppe Seylers Z Physiol Chem. 1976 Nov;357(11):1469-76. doi: 10.1515/bchm2.1976.357.2.1469.
Rat cerebral cortex slices were incubated with 1muM [3H]4-aminobutyrate or [3H]L-glutamine. The subcellular distribution of the accumulated labelled substances were determined by fractionating the nuclei-free homogenates on a 5-step discontinuous sucrose density gradient in a B XIV zonal rotor. The gradient was designed to separate the synaptosomes into 3 subpopulations of increasing density. The patterns of distribution of [3H]4-aminobutyrate and [3H-a1L-glutamate in the three synaptosomal peaks were distinctly different. This indicates the presence of separate types of nerve ending accumulating these two potential neurotransmitters, which are known to be metabolically closely linked through the enzyme glutamate decarboxylase. Recentrifugation of the 3 synaptosomal peaks on flat step gradients in a swingout rotor did not result in any further enrichment in transmitter-specific synaptosomes.
将大鼠大脑皮层切片与1μM [3H]4-氨基丁酸或[3H]L-谷氨酰胺一起孵育。通过在B XIV区带转子中在5步不连续蔗糖密度梯度上对无核匀浆进行分级分离，来确定积累的标记物质的亚细胞分布。该梯度旨在将突触体分离成密度递增的3个亚群。[3H]4-氨基丁酸和[3H]L-谷氨酸在三个突触体峰中的分布模式明显不同。这表明存在积累这两种潜在神经递质的不同类型的神经末梢，已知它们通过谷氨酸脱羧酶在代谢上紧密相连。在摆动式转子中的平阶梯度上对3个突触体峰进行再离心，并未导致递质特异性突触体的进一步富集。
