中老年男性脂肪组织脂蛋白脂肪酶的预测因素：与瘦素和肥胖的关系，而非与心血管健康的关系。

Predictors of adipose tissue lipoprotein lipase in middle-aged and older men: relationship to leptin and obesity, but not cardiovascular fitness.

作者信息

Berman D M, Rogus E M, Busby-Whitehead M J, Katzel L I, Goldberg A P

机构信息

Department of Medicine, University of Maryland School of Medicine, Baltimore, USA.

出版信息

Metabolism. 1999 Feb;48(2):183-9. doi: 10.1016/s0026-0495(99)90031-8.

DOI:10.1016/s0026-0495(99)90031-8

PMID:10024079

Abstract

The effects of long-term endurance exercise training, body composition, and cardiovascular fitness (VO2max) on the activity of adipose tissue lipoprotein lipase (AT-LPL) and lipoprotein lipids were examined in 66 healthy age-matched middle-aged and older men (mean +/- SE, 61 +/- 1 years). We compared subcutaneous abdominal (ABD) and gluteal (GLT) heparin-elutable AT-LPL activity in 19 master athletes (VO2max > 40 mL/kg/min) and 20 lean sedentary men (VO2max < 40 mL/kg/min) versus 27 obese sedentary men (VO2max < 40 mL/kg/min; body fat > 27%). Fasting insulin and leptin levels were similar in master athletes and lean sedentary men, but were lower than in obese sedentary men. There were no differences in fasting values for total cholesterol or low-density lipoprotein cholesterol (LDL-C) among the groups, but master athletes had lower triglyceride (TG) values (P < .05) and higher high-density lipoprotein cholesterol (HDL-C) and HDL2-C (P < .05) than obese and lean sedentary men. There were no regional (ABD v GLT) differences in the activity of AT-LPL in these groups, but obese sedentary men had higher levels of ABD AT-LPL (2.1 +/- 0.3 nmol/10(6) cells x min) than lean sedentary men (0.8 +/- 0.2) and master athletes (0.5 +/- 0.1, P = .01). Similar results were observed for GLT AT-LPL. Both ABD and GLT AT-LPL activity correlated positively with percent body fat (r = .46 to .54, P < .001), fasting insulin (r = .37 to .45, P < .001), and leptin (r = .61 to .65, P < .0001), but not with VO2max. In stepwise multiple regression analysis, leptin was the main independent predictor of ABD (R2 = .43, P < .0001) and GLT (R2 = .40, P < .0001) AT-LPL activity. Plasma TG correlated positively (r = .32, P < .01) and HDL-C correlated negatively (r = -.32, P = .02) with ABD AT-LPL activity, but these relationships were not significant after controlling for percent body fat or leptin. The results of this study indicate that in healthy middle-aged and older men, the major determinants of AT-LPL activity are obesity and its major associated hormones, leptin and insulin, not cardiovascular fitness, and also suggest that the higher HDL-C levels observed in endurance-trained men are not associated with increased AT-LPL activity.

摘要

在66名年龄匹配的健康中年及老年男性（平均±标准误，61±1岁）中，研究了长期耐力运动训练、身体成分和心血管适能（最大摄氧量）对脂肪组织脂蛋白脂肪酶（AT-LPL）活性及脂蛋白脂质的影响。我们比较了19名大师级运动员（最大摄氧量>40 mL/kg/min）、20名体型瘦的久坐男性（最大摄氧量<40 mL/kg/min）与27名肥胖久坐男性（最大摄氧量<40 mL/kg/min；体脂>27%）的腹部皮下（ABD）和臀肌（GLT）肝素可洗脱AT-LPL活性。大师级运动员和体型瘦的久坐男性的空腹胰岛素和瘦素水平相似，但低于肥胖久坐男性。各组间总胆固醇或低密度脂蛋白胆固醇（LDL-C）的空腹值无差异，但大师级运动员的甘油三酯（TG）值较低（P<.05），高密度脂蛋白胆固醇（HDL-C）和HDL2-C较高（P<.05），高于肥胖和体型瘦的久坐男性。这些组中AT-LPL活性无区域（ABD与GLT）差异，但肥胖久坐男性的ABD AT-LPL水平（2.1±0.3 nmol/10⁶细胞×分钟）高于体型瘦的久坐男性（0.8±0.2）和大师级运动员（0.5±0.1，P=.01）。GLT AT-LPL也观察到类似结果。ABD和GLT AT-LPL活性均与体脂百分比呈正相关（r=.46至.54，P<.001）、空腹胰岛素（r=.37至.45，P<.001）和瘦素（r=.61至.65，P<.0001），但与最大摄氧量无关。在逐步多元回归分析中，瘦素是ABD（R²=.43，P<.0001）和GLT（R²=.40，P<.0001）AT-LPL活性的主要独立预测因子。血浆TG与ABD AT-LPL活性呈正相关（r=.32，P<.01），HDL-C与ABD AT-LPL活性呈负相关（r=-.32，P=.02），但在控制体脂百分比或瘦素后，这些关系不显著。本研究结果表明，在健康中年及老年男性中，AT-LPL活性的主要决定因素是肥胖及其主要相关激素瘦素和胰岛素，而非心血管适能，还表明耐力训练男性中观察到的较高HDL-C水平与AT-LPL活性增加无关。