Zughaier S M, Ryley H C, Jackson S K
Department of Medical Microbiology, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, United Kingdom.
Infect Immun. 1999 Mar;67(3):1505-7. doi: 10.1128/IAI.67.3.1505-1507.1999.
Whole cells and lipopolysaccharides (LPSs) extracted from Burkholderia cepacia, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (TNF-alpha) from the human monocyte cell line MonoMac-6. B. cepacia LPS, on a weight-for-weight basis, was found to have TNF-alpha-inducing activity similar to that of LPS from E. coli, which was approximately four- and eightfold greater than the activity of LPSs from P. aeruginosa and S. maltophilia, respectively. The LPS-stimulated TNF-alpha production from monocytes was found to be CD14 dependent. These results suggest that B. cepacia LPS might play a role in the pathogenesis of inflammatory lung disease in cystic fibrosis, and in some patients it might be responsible, at least in part, for the sepsis-like cepacia syndrome.
对从洋葱伯克霍尔德菌、铜绿假单胞菌、嗜麦芽窄食单胞菌和大肠杆菌中提取的全细胞和脂多糖（LPS）刺激人单核细胞系MonoMac-6产生肿瘤坏死因子α（TNF-α）的能力进行了比较。发现按重量计算，洋葱伯克霍尔德菌LPS诱导TNF-α的活性与大肠杆菌LPS相似，分别比铜绿假单胞菌和嗜麦芽窄食单胞菌LPS的活性高约四倍和八倍。发现LPS刺激单核细胞产生TNF-α是依赖CD14的。这些结果表明，洋葱伯克霍尔德菌LPS可能在囊性纤维化炎症性肺病的发病机制中起作用，在一些患者中，它可能至少部分地导致了类似败血症的洋葱伯克霍尔德菌综合征。
