铜绿假单胞菌外毒素S是一种双谷氨酸ADP核糖基转移酶。
Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase.
作者信息
Radke J, Pederson K J, Barbieri J T
机构信息
Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
出版信息
Infect Immun. 1999 Mar;67(3):1508-10. doi: 10.1128/IAI.67.3.1508-1510.1999.
Abstract
Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein.
摘要
铜绿假单胞菌外毒素S（ExoS）中两个突变的动力学分析表明，E379D突变抑制了ADP核糖基转移酶活性的表达，但对NAD糖水解酶活性的表达影响很小，而E381D突变则抑制了这两种活性的表达。这些数据确定ExoS为双谷氨酸ADP核糖基转移酶，其中E381是催化残基，E379有助于将ADP核糖转移到靶蛋白上。
相似文献
1
Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase.铜绿假单胞菌外毒素S是一种双谷氨酸ADP核糖基转移酶。
Infect Immun. 1999 Mar;67(3):1508-10. doi: 10.1128/IAI.67.3.1508-1510.1999.
2
Identification of glutamic acid 381 as a candidate active site residue of Pseudomonas aeruginosa exoenzyme S.鉴定谷氨酸381为铜绿假单胞菌外毒素S的候选活性位点残基。
Biochemistry. 1996 Feb 27;35(8):2754-8. doi: 10.1021/bi952340g.
3
Expression of FAS-independent ADP-ribosyltransferase activity by a catalytic deletion peptide of Pseudomonas aeruginosa exoenzyme S.铜绿假单胞菌外毒素S的催化缺失肽表现出不依赖FAS的ADP核糖基转移酶活性。
Biochemistry. 1999 May 4;38(18):5858-63. doi: 10.1021/bi982133r.
4
Molecular heterogeneity of a type III cytotoxin, Pseudomonas aeruginosa exoenzyme S.III型细胞毒素铜绿假单胞菌外毒素S的分子异质性
Biochemistry. 2003 Dec 9;42(48):14249-57. doi: 10.1021/bi035053i.
5
Auto-ADP-ribosylation of Pseudomonas aeruginosa ExoS.铜绿假单胞菌外毒素S的自动ADP核糖基化
J Biol Chem. 2002 Apr 5;277(14):12082-8. doi: 10.1074/jbc.M109039200. Epub 2002 Jan 30.
6
Membrane localization contributes to the in vivo ADP-ribosylation of Ras by Pseudomonas aeruginosa ExoS.膜定位有助于铜绿假单胞菌外毒素S在体内对Ras进行ADP核糖基化修饰。
Infect Immun. 2002 Apr;70(4):2230-2. doi: 10.1128/IAI.70.4.2230-2232.2002.
7
Intracellular expression of the ADP-ribosyltransferase domain of Pseudomonas exoenzyme S is cytotoxic to eukaryotic cells.铜绿假单胞菌外毒素S的ADP核糖基转移酶结构域在细胞内的表达对真核细胞具有细胞毒性。
Mol Microbiol. 1998 Nov;30(4):751-9. doi: 10.1046/j.1365-2958.1998.01106.x.
8
Biochemical relationships between the 53-kilodalton (Exo53) and 49-kilodalton (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa.铜绿假单胞菌外切酶S的53千道尔顿（Exo53）和49千道尔顿（ExoS）形式之间的生化关系。
J Bacteriol. 1997 Mar;179(5):1609-13. doi: 10.1128/jb.179.5.1609-1613.1997.
9
Ecto-ADP-ribosyltransferase activity of Pseudomonas aeruginosa exoenzyme S.铜绿假单胞菌外毒素S的胞外ADP核糖基转移酶活性
Infect Immun. 1997 Aug;65(8):3304-9. doi: 10.1128/iai.65.8.3304-3309.1997.
10
Independent and coordinate effects of ADP-ribosyltransferase and GTPase-activating activities of exoenzyme S on HT-29 epithelial cell function.外毒素S的ADP核糖基转移酶和GTP酶激活活性对HT-29上皮细胞功能的独立及协同作用。
Infect Immun. 2001 Sep;69(9):5318-28. doi: 10.1128/IAI.69.9.5318-5328.2001.
引用本文的文献
1
Novel humanized anti-PcrV monoclonal antibody COT-143 protects mice from lethal infection via inhibition of toxin translocation by the type III secretion system.新型人源化抗 PcrV 单克隆抗体 COT-143 通过抑制 III 型分泌系统的毒素转位保护小鼠免受致死性感染。
Antimicrob Agents Chemother. 2024 Oct 8;68(10):e0069424. doi: 10.1128/aac.00694-24. Epub 2024 Sep 13.
2
The Role of ExoS in Dissemination of Pseudomonas aeruginosa during Pneumonia.外毒素S在铜绿假单胞菌肺炎传播中的作用
PLoS Pathog. 2015 Jun 19;11(6):e1004945. doi: 10.1371/journal.ppat.1004945. eCollection 2015 Jun.
3
The ADP-ribosyltransferase domain of the effector protein ExoS inhibits phagocytosis of Pseudomonas aeruginosa during pneumonia.效应蛋白ExoS的ADP核糖基转移酶结构域在肺炎期间抑制铜绿假单胞菌的吞噬作用。
mBio. 2014 Jun 10;5(3):e01080-14. doi: 10.1128/mBio.01080-14.
4
Exoenzyme S ADP-ribosylates Rab5 effector sites to uncouple intracellular trafficking.外切酶 S 通过 ADP-ribosylates Rab5 效应物位点来解偶联细胞内运输。
Infect Immun. 2014 Jan;82(1):21-8. doi: 10.1128/IAI.01059-13. Epub 2013 Oct 7.
5
The opportunistic pathogen Pseudomonas aeruginosa activates the DNA double-strand break signaling and repair pathway in infected cells.机会性病原体铜绿假单胞菌在感染细胞中激活 DNA 双链断裂信号转导和修复途径。
Cell Mol Life Sci. 2013 Nov;70(22):4385-97. doi: 10.1007/s00018-013-1392-3. Epub 2013 Jun 13.
6
Structure and function of the Type III secretion system of Pseudomonas aeruginosa.铜绿假单胞菌 III 型分泌系统的结构与功能。
Curr Protein Pept Sci. 2012 Dec;13(8):831-42. doi: 10.2174/138920312804871210.
7
Translocon-independent intracellular replication by Pseudomonas aeruginosa requires the ADP-ribosylation domain of ExoS.铜绿假单胞菌的转位器非依赖性细胞内复制需要 ExoS 的 ADP-核糖基化结构域。
Microbes Infect. 2012 Dec;14(15):1366-73. doi: 10.1016/j.micinf.2012.08.007. Epub 2012 Aug 30.
8
Host cell cytotoxicity and cytoskeleton disruption by CerADPr, an ADP-ribosyltransferase of Bacillus cereus G9241.蜡状芽孢杆菌 G9241 的 ADP-ribosyltransferase CerADPr 引起的宿主细胞细胞毒性和细胞骨架破坏。
Biochemistry. 2013 Apr 2;52(13):2309-18. doi: 10.1021/bi300692g. Epub 2013 Mar 20.
9
Clostridium perfringens iota-toxin: structure and function.产气荚膜梭菌 ι 型毒素：结构与功能。
Toxins (Basel). 2009 Dec;1(2):208-28. doi: 10.3390/toxins1020208. Epub 2009 Dec 23.
10
The ADP-ribosylation domain of Pseudomonas aeruginosa ExoS is required for membrane bleb niche formation and bacterial survival within epithelial cells.铜绿假单胞菌 ExoS 的 ADP-ribosylation 结构域对于在质膜泡形成和细菌在上皮细胞内的存活是必需的。
Infect Immun. 2010 Nov;78(11):4500-10. doi: 10.1128/IAI.00417-10. Epub 2010 Aug 23.
本文引用的文献
1
The amino-terminal domain of Pseudomonas aeruginosa ExoS disrupts actin filaments via small-molecular-weight GTP-binding proteins.铜绿假单胞菌外毒素S的氨基末端结构域通过小分子GTP结合蛋白破坏肌动蛋白丝。
Mol Microbiol. 1999 Apr;32(2):393-401. doi: 10.1046/j.1365-2958.1999.01359.x.
2
Intracellular expression of the ADP-ribosyltransferase domain of Pseudomonas exoenzyme S is cytotoxic to eukaryotic cells.铜绿假单胞菌外毒素S的ADP核糖基转移酶结构域在细胞内的表达对真核细胞具有细胞毒性。
Mol Microbiol. 1998 Nov;30(4):751-9. doi: 10.1046/j.1365-2958.1998.01106.x.
3
The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton.鼠伤寒沙门氏菌酪氨酸磷酸酶SptP被转运到宿主细胞中并破坏肌动蛋白细胞骨架。
Mol Microbiol. 1998 Jan;27(2):359-68. doi: 10.1046/j.1365-2958.1998.00684.x.
4
Infections with Pseudomonas aeruginosa in patients with cystic fibrosis.囊性纤维化患者的铜绿假单胞菌感染
Behring Inst Mitt. 1997 Feb(98):249-55.
5
Intracellular targeting of exoenzyme S of Pseudomonas aeruginosa via type III-dependent translocation induces phagocytosis resistance, cytotoxicity and disruption of actin microfilaments.铜绿假单胞菌外毒素S通过III型依赖易位进行细胞内靶向作用，可诱导吞噬抵抗、细胞毒性以及肌动蛋白微丝的破坏。
Mol Microbiol. 1997 Sep;25(6):1125-39. doi: 10.1046/j.1365-2958.1997.5411905.x.
6
Biochemical relationships between the 53-kilodalton (Exo53) and 49-kilodalton (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa.铜绿假单胞菌外切酶S的53千道尔顿（Exo53）和49千道尔顿（ExoS）形式之间的生化关系。
J Bacteriol. 1997 Mar;179(5):1609-13. doi: 10.1128/jb.179.5.1609-1613.1997.
7
Glutamic acid 207 in rodent T-cell RT6 antigens is essential for arginine-specific ADP-ribosylation.啮齿动物T细胞RT6抗原中的谷氨酸207对于精氨酸特异性ADP核糖基化至关重要。
J Biol Chem. 1996 Nov 22;271(47):29552-5. doi: 10.1074/jbc.271.47.29552.
8
Evidence that Arg-295, Glu-378, and Glu-380 are active-site residues of the ADP-ribosyltransferase activity of iota toxin.精氨酸-295、谷氨酸-378和谷氨酸-380是埃托毒素ADP核糖基转移酶活性的活性位点残基的证据。
FEBS Lett. 1996 Oct 21;395(2-3):191-4. doi: 10.1016/0014-5793(96)01035-6.
9
Genetic relationship between the 53- and 49-kilodalton forms of exoenzyme S from Pseudomonas aeruginosa.铜绿假单胞菌外毒素S的53千道尔顿和49千道尔顿形式之间的遗传关系。
J Bacteriol. 1996 Mar;178(5):1412-9. doi: 10.1128/jb.178.5.1412-1419.1996.
10
Identification of glutamic acid 381 as a candidate active site residue of Pseudomonas aeruginosa exoenzyme S.鉴定谷氨酸381为铜绿假单胞菌外毒素S的候选活性位点残基。
Biochemistry. 1996 Feb 27;35(8):2754-8. doi: 10.1021/bi952340g.
Zotero 插件