人体心肌pH值的饱和转移测量法。

Measurement of myocardial pH by saturation transfer in man.

作者信息

Blamire A M, Rajagopalan B, Radda G K

机构信息

MRC Biochemical and Clinical Magnetic Resonance Spectroscopy Unit, John Radcliffe Hospital, Headington, Oxford, England.

出版信息

Magn Reson Med. 1999 Jan;41(1):198-203. doi: 10.1002/(sici)1522-2594(199901)41:1<198::aid-mrm28>3.0.co;2-h.

DOI:10.1002/(sici)1522-2594(199901)41:1<198::aid-mrm28>3.0.co;2-h

PMID:10025630

Abstract

Myocardial pH has been shown in animal models to be a sensitive indicator of ischemia. In vivo measurement in humans using 31p magnetic resonance spectroscopy is complicated by the overlap of blood 2,3-diphosphoglycerate peaks with the P(i) peak used for pH measurement. A "saturation transfer" method combined with spatial presaturation of skeletal muscle signal is presented which can obtain spectra from the heart free of contamination of 2,3-DPG signal in which intracellular P(i) resonance can be clearly observed. Application to a group of six normal subjects found that the chemical shift of the intracellular inorganic phosphate peak was 4.95+/-0.06 relative to the phosphocreatine peak. This is equivalent to a pH of 7.11+/-0.05.

摘要

在动物模型中，心肌pH已被证明是缺血的敏感指标。在人体中使用31P磁共振波谱进行体内测量时，血液中2,3-二磷酸甘油酸峰与用于pH测量的无机磷酸（P(i)）峰重叠，使测量变得复杂。本文提出了一种“饱和转移”方法，并结合骨骼肌信号的空间预饱和，该方法可获取不受2,3-二磷酸甘油酸信号污染的心脏波谱，其中细胞内无机磷酸共振可清晰观察到。对一组6名正常受试者的应用发现，细胞内无机磷酸峰相对于磷酸肌酸峰的化学位移为4.95±0.06。这相当于pH值为7.11±0.05。