通过高效液相色谱法对其苯甲酰衍生物进行葡糖神经酰胺分子种类的分离。
Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives.
作者信息
Suzuki A, Handa S, Yamakawa T
出版信息
J Biochem. 1976 Nov;80(5):1181-3. doi: 10.1093/oxfordjournals.jbchem.a131375.
Abstract
The method of separation of glucosylceramide by HPLC was reported. Glucosylceramide was perbenzoylated and separated on a packed muBondapack C18 column, using methanol as eluting solvent. The pattern obtained by HPLC closely resembled that obtained by GLC of the TMS-glucosylceramide, and reflected the molecular species of fatty acid components. This method is reproducible, and sensitive as GLC. This method also can be used for analysis of higher glycolipids.
摘要
报道了用高效液相色谱法分离葡糖神经酰胺的方法。葡糖神经酰胺经全苯甲酰化后，在装填有μBondapack C18的柱上进行分离，用甲醇作为洗脱溶剂。高效液相色谱法得到的图谱与三甲基硅烷基葡糖神经酰胺的气相色谱法得到的图谱非常相似，反映了脂肪酸成分的分子种类。该方法具有可重复性，且与气相色谱法一样灵敏。该方法也可用于分析高级糖脂。
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Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives.通过高效液相色谱法对其苯甲酰衍生物进行葡糖神经酰胺分子种类的分离。
J Biochem. 1976 Nov;80(5):1181-3. doi: 10.1093/oxfordjournals.jbchem.a131375.
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