Kaye E M, Ullman M D
Anal Biochem. 1984 May 1;138(2):380-5. doi: 10.1016/0003-2697(84)90825-x.
Perbenzoylated gluco- and galactocerebrosides were separated and quantitated with a modification of existing HPLC methodology. A linear gradient of 0.9-18.4% dioxane in hexane was used to separate the derivatives on a pellicular silica column heated to 65 degrees C. The elevated temperature was the major modification that permitted the separation. This system was compared to a previously reported silica column/isopropanol in hexane system.
经苯甲酰化的葡萄糖脑苷脂和半乳糖脑苷脂通过对现有高效液相色谱法进行改进后得以分离和定量。采用在己烷中含0.9 - 18.4%二氧六环的线性梯度洗脱，在加热至65℃的薄壳硅胶柱上分离这些衍生物。升高温度是实现分离的主要改进措施。该系统与先前报道的硅胶柱/己烷中的异丙醇系统进行了比较。
