Wang C X, Yang H, Perrott C J, Gietzen D W
Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California at Davis, 95616, USA.
Neurosci Lett. 1999 Jan 4;259(1):53-5. doi: 10.1016/s0304-3940(98)00873-8.
Effects of dietary amino acid deficiency on interstitial levels of norepinephrine (NE) were assessed in the ventromedial hypothalamic nucleus (VMH). Microdialysates, collected from the VMH, were analyzed using high pressure liquid chromatography with electrochemical detection (HPLC-EC). Ingestion of an amino acid imbalanced diet, which causes a rapid deficiency of the limiting amino acid, induced a significant decrease in the NE concentration from the VMH. The changes in the NE concentration appeared 60 min after diet ingestion and the lowest NE level was observed at 180 min. The present results suggest that ingestion of an amino acid imbalanced diet inhibits NE release in the VMH and support the hypothesis that the VMH plays a role in the integration of signals for the feeding responses to changes in essential amino acid availability.
评估了膳食氨基酸缺乏对下丘脑腹内侧核（VMH）中去甲肾上腺素（NE）间质水平的影响。从VMH收集的微透析液采用电化学检测高压液相色谱法（HPLC-EC）进行分析。摄入导致限制性氨基酸迅速缺乏的氨基酸不平衡饮食，会使VMH中的NE浓度显著降低。NE浓度的变化在饮食摄入后60分钟出现，在180分钟时观察到最低NE水平。目前的结果表明，摄入氨基酸不平衡饮食会抑制VMH中NE的释放，并支持以下假设：VMH在整合对必需氨基酸可用性变化的进食反应信号中发挥作用。
