Torimura M, Ito S, Kano K, Ikeda T, Esaka Y, Ueda T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
J Chromatogr B Biomed Sci Appl. 1999 Jan 8;721(1):31-7. doi: 10.1016/s0378-4347(98)00490-3.
Capillary zone electrophoresis (CZE) was applied to the electrophoretic characterization for microorganisms. The electrophoretic peaks detected using light scattering phenomena were characteristic of the microorganisms used. The electrophoretic mobility (mu) evaluated by CZE was in good agreement with that obtained by classical electrophoresis of microorganisms. The migration time was reproducible and depended on the ionic strength (I). Analysis of the mu vs. I relationship provided information regarding the charge density and the hardness of the microbial cell surface. The redox enzymatic activity of microorganisms was also evaluated by CZE using a running buffer containing a corresponding substrate and an appropriate exogenous electron acceptor. A decrease in the concentration of the electron acceptor due to microbial activity can be simultaneously monitored during the electrophoretic process without significant modification of the CZE instrument. Effects of some chemical treatments of microbial cells were also studied using this technique.
毛细管区带电泳（CZE）被应用于微生物的电泳表征。利用光散射现象检测到的电泳峰是所用微生物的特征峰。通过CZE评估的电泳迁移率（μ）与通过微生物经典电泳获得的迁移率高度一致。迁移时间具有可重复性，并且取决于离子强度（I）。对μ与I关系的分析提供了有关微生物细胞表面电荷密度和硬度的信息。微生物的氧化还原酶活性也通过CZE使用含有相应底物和适当外源电子受体的运行缓冲液进行评估。在电泳过程中，可以同时监测由于微生物活性导致的电子受体浓度降低，而无需对CZE仪器进行重大改装。还使用该技术研究了微生物细胞的一些化学处理效果。
