Fredriksen B, Press C M, Løken T, Odegaard S A
Department of Reproduction and Forensic Medicine, Norwegian College of Veterinary Medicine, Oslo, Norway.
Vet Microbiol. 1999 Jan;64(2-3):109-22. doi: 10.1016/s0378-1135(98)00263-6.
The tissue distribution and cellular localisation of bovine virus diarrhoea virus (BVDV) was investigated in the uterus, placentomes, intercotyledonary foetal membranes and foetal organs of three persistently infected (PI) pregnant heifers. The uterus and ovaries of a non-pregnant PI heifer were also included in the study. Cryostat sections were examined using immunohistochemical techniques and monoclonal antibodies against BVDV. A double immunofluorescence technique was used to identify BVDV positive cells that also showed staining for either the leukocyte common antigen CD45 or the cytoskeletal filament vimentin. BVDV antigen was detected in all the organs examined, and was present in both epithelial and non-epithelial cells. In all organs many of the virus-positive cells also showed reactivity for vimentin. In the foetal liver and spleen a small, scattered population of virus-positive cells showed reactivity for CD45. A few cells showed reactivity both for BVDV antigen and for CD45 in the placentomes and intercotyledonary foetal membranes. In contrast to earlier reports, only scattered cells in the foetal part of the placentomes, the cotyledons, showed reactivity for BVDV antigen. However, in the chorion of the intercotyledonary foetal membranes, a larger proportion of the trophoblast cells showed reactivity for BVDV, especially the binuclear trophoblast cells. In the uterus, pregnancy appeared to favour virus replication, as the section from the pregnant heifers showed much stronger staining and a higher proportion of viral antigen-positive cells than sections from the non-pregnant PI heifer.
对三头持续感染（PI）的怀孕小母牛的子宫、胎盘、绒毛间隙胎膜和胎儿器官中牛病毒性腹泻病毒（BVDV）的组织分布和细胞定位进行了研究。研究还纳入了一头未怀孕的PI小母牛的子宫和卵巢。使用免疫组织化学技术和抗BVDV单克隆抗体对冰冻切片进行检查。采用双重免疫荧光技术鉴定同时对白细胞共同抗原CD45或细胞骨架细丝波形蛋白呈阳性染色的BVDV阳性细胞。在所有检查的器官中均检测到BVDV抗原，且其存在于上皮细胞和非上皮细胞中。在所有器官中，许多病毒阳性细胞也对波形蛋白呈反应性。在胎儿肝脏和脾脏中，一小部分散在的病毒阳性细胞对CD45呈反应性。在胎盘和绒毛间隙胎膜中有少数细胞对BVDV抗原和CD45均呈反应性。与早期报告相反，胎盘胎儿部分（子叶）中仅散在细胞对BVDV抗原呈反应性。然而，在绒毛间隙胎膜的绒毛膜中，较大比例的滋养层细胞对BVDV呈反应性，尤其是双核滋养层细胞。在子宫中，怀孕似乎有利于病毒复制，因为怀孕小母牛的切片显示出比未怀孕的PI小母牛的切片更强的染色和更高比例的病毒抗原阳性细胞。
