Falsen E, Pascual C, Sjödén B, Ohlén M, Collins M D
Department of Clinical Bacteriology, University of Göteborg, Sweden.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:217-21. doi: 10.1099/00207713-49-1-217.
Eleven strains of a hitherto undescribed Gram-positive, catalase-negative, facultatively anaerobic rod-shaped bacterium from human sources and medical care products were characterized by phenotypic and molecular taxonomic methods. The phenotypic properties of the bacterium were consistent with its assignment to the genus Lactobacillus but it was readily distinguished from all currently described species of this genus by its biochemical characteristics and by SDS-PAGE analysis of its cellular proteins. Comparative 16S rRNA gene sequence analysis demonstrated that the unknown bacterium was a member of rRNA group I Lactobacillus which includes Lactobacillus delbrueckii, the type species of the genus, and close relatives. Lactobacillus gasseri and Lactobacillus johnsonii were the nearest phylogenetic relatives of the unknown bacterium, but 16S rRNA sequence divergence values of > 4% clearly showed that it represents a distinct species. Based on both phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium should be classified in the genus Lactobacillus, as Lactobacillus iners sp. nov. The type strain of Lactobacillus iners is CCUG 28746T.
通过表型和分子分类学方法对11株来自人类样本和医疗产品的革兰氏阳性、过氧化氢酶阴性、兼性厌氧的杆状细菌进行了特征描述，该细菌此前未被描述过。该细菌的表型特征与其归属于乳杆菌属一致，但通过其生化特性和细胞蛋白的SDS-PAGE分析，很容易与该属目前已描述的所有物种区分开来。比较16S rRNA基因序列分析表明，这种未知细菌是rRNA第一组乳杆菌的成员，该组包括该属的模式种德氏乳杆菌及其近缘种。加氏乳杆菌和约翰逊乳杆菌是该未知细菌最近的系统发育亲缘种，但16S rRNA序列差异值>4%清楚地表明它代表一个独特的物种。基于系统发育和表型证据，建议将该未知细菌归为乳杆菌属，命名为惰性乳杆菌（Lactobacillus iners sp. nov.）。惰性乳杆菌的模式菌株为CCUG 28746T。
