Blackall P J, Bowles R, Pahoff J L, Smith B N
Australasian Pig Institute, Queensland Department of Primary Industries, Yeerongpilly.
Aust Vet J. 1999 Jan;77(1):39-43. doi: 10.1111/j.1751-0813.1999.tb12426.x.
To clarify the serological identity of the prototype strain of a group of Actinobacillus pleuropneumoniae isolates that could not be serotyped in previous studies and to establish the serovar of 378 isolates of A pleuropneumoniae obtained from pigs in Australia over the period 1993 to 1996.
After initial validation, QGD and IHA tests were used to characterise the prototype isolate (HS143) selected to represent the cross-reacting isolates that were found in a previous study. Next, 378 recent field isolates of A pleuropneumoniae were characterised using the existing gel diffusion serotyping technique and/or the IHA or QGD tests.
The indirect haemagglutination test was shown to be capable of correctly recognising the reference strain for all serovars except serovar 11. While the quantitative gel diffusion test was not as effective as indirect haemagglutination, it could recognise serovar 11. When the two tests were used to examine the prototype strain (HS143) of the cross-reactive isolates, the results indicated that HS143 is serologically distinct from all 12 of the recognised serovars of A pleuropneumoniae. However, as HS143 was subsequently identified as serovar 12 by one of the leading international reference laboratories, the antiserum to isolate HS143 was used as the serovar 12 antiserum. A total of 346 of the 378 A pleuropneumoniae field isolates examined could be confidently serotyped with almost 90% of the isolates being either serovar 1 (104 isolates); serovar 7 (83 isolates) or serovar 12 (142 isolates). A range of other serovars and some cross-reactive isolates made up the remainder of the isolates.
The serovar 12 antiserum produced against the international reference strain (1096) does not recognise Australian serovar 12 isolates. The antiserum raised against isolate HS143 does recognise the Australian serovar 12 isolates. The dominant serovars of A pleuropneumoniae infecting Australian pigs are (in decreasing order) serovars 12, 1 and 7.
明确一组胸膜肺炎放线杆菌分离株原型菌株的血清学特性，这些分离株在以往研究中无法进行血清分型，并确定1993年至1996年期间从澳大利亚猪群中获得的378株胸膜肺炎放线杆菌的血清型。
在初步验证后，采用定量凝胶扩散试验（QGD）和间接血凝试验（IHA）对所选代表先前研究中发现的交叉反应性分离株的原型分离株（HS143）进行特性鉴定。接下来，使用现有的凝胶扩散血清分型技术和/或IHA或QGD试验对378株近期胸膜肺炎放线杆菌野外分离株进行特性鉴定。
间接血凝试验能够正确识别除血清型11以外的所有血清型的参考菌株。虽然定量凝胶扩散试验不如间接血凝试验有效，但它能够识别血清型11。当使用这两种试验检测交叉反应性分离株的原型菌株（HS143）时，结果表明HS143在血清学上与胸膜肺炎放线杆菌所有12种已识别的血清型不同。然而，由于HS1后来被一家领先的国际参考实验室鉴定为血清型12，因此针对分离株HS143的抗血清被用作血清型12抗血清。在检测的378株胸膜肺炎放线杆菌野外分离株中，共有346株能够可靠地进行血清分型，其中近90%的分离株为血清型1（104株）、血清型7（83株）或血清型12（142株）。一系列其他血清型和一些交叉反应性分离株构成了其余的分离株。
针对国际参考菌株（1096）产生的血清型12抗血清不能识别澳大利亚血清型12分离株。针对分离株HS143产生的抗血清能够识别澳大利亚血清型12分离株。感染澳大利亚猪的胸膜肺炎放线杆菌的优势血清型依次为血清型12、1和7。
