Yang M, Jiang P, Sun F X, Hasegawa S, Baranov E, Chishima T, Shimada H, Moossa A R, Hoffman R M
AntiCancer, Inc., San Diego, California 92111, USA.
Cancer Res. 1999 Feb 15;59(4):781-6.
Here, we report a fluorescent spontaneous bone metastatic model of human prostate cancer developed by surgical orthotopic implantation of green fluorescent protein (GFP)-expressing prostate cancer tissue. Human prostate cancer PC-3 cells were transduced with the pLEIN expression retroviral vector containing the enhanced GFP and neomycin resistance genes. Stable GFP high-expression PC-3 clones were selected in vitro with G418, which were then combined and injected s.c. in nude mice. For metastasis studies, fragments of a single highly fluorescent s.c. growing tumor were implanted by surgical orthotopic implantation in the prostate of a series of nude mice. Subsequent micrometastases and metastases were visualized by GFP fluorescence throughout the skeleton, including the skull, rib, pelvis, femur, and tibia The central nervous system, including the brain and spinal cord, was also involved with tumor, as visualized by GFP fluorescence. Systemic organs, including the lung, plural membrane, liver, kidney, and adrenal gland, also had fluorescent metastases. The metastasis pattern in this model reflects the bone and other metastatic sites of human prostate cancer. Thus, this model should be very useful for the study and development of treatment for metastatic androgen-independent prostate cancer.
在此，我们报告一种通过手术原位植入表达绿色荧光蛋白（GFP）的前列腺癌组织建立的人前列腺癌荧光自发骨转移模型。用人前列腺癌PC-3细胞转导含有增强型GFP和新霉素抗性基因的pLEIN表达逆转录病毒载体。在体外使用G418筛选出稳定的GFP高表达PC-3克隆，然后将其合并并皮下注射到裸鼠体内。为了进行转移研究，通过手术原位植入将单个高度荧光的皮下生长肿瘤的片段植入一系列裸鼠的前列腺中。随后通过GFP荧光在整个骨骼（包括颅骨、肋骨、骨盆、股骨和胫骨）中观察到微转移和转移灶。中枢神经系统（包括脑和脊髓）也有肿瘤累及，通过GFP荧光可见。包括肺、胸膜、肝脏、肾脏和肾上腺在内的全身器官也有荧光转移灶。该模型中的转移模式反映了人前列腺癌的骨转移和其他转移部位。因此，该模型对于转移性雄激素非依赖性前列腺癌的治疗研究和开发应该非常有用。
