一种可无创成像的 GFP 表达的人膀胱癌原位模型鼠。
A Non-invasive Imageable GFP-expressing Mouse Model of Orthotopic Human Bladder Cancer.
机构信息
AntiCancer, Inc., San Diego, CA, U.S.A.
Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
出版信息
In Vivo. 2020 Nov-Dec;34(6):3225-3231. doi: 10.21873/invivo.12158.
BACKGROUND/AIM: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant human cancer.
MATERIALS AND METHODS
A highly-fluorescent green fluorescent protein (GFP)-expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 human bladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box.
RESULTS
The GFP-expressing orthotopic bladder tumor was imaged in real-time non-invasively as well as intra-vitally, with the two methods correlating at r=0.99.
CONCLUSION
This is the first non-invasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease.
背景/目的：为了开发膀胱癌的有效药物，我们需要建立更逼真的膀胱癌小鼠模型。通过增强明亮的荧光报告基因来实时跟踪疾病的肿瘤模型，将提高准确预测各种治疗方法对这种特别恶性人类癌症疗效的能力。
材料和方法
使用人膀胱癌细胞系 UM-UC-3（UM-UC-3-GFP），通过将 GFP 表达的膀胱癌模型原位建立在裸鼠中。将 UM-UC-3-GFP 皮下肿瘤的片段通过手术植入裸鼠膀胱中。使用简单的成像盒获得非侵入性和活体荧光成像。
结果
实时非侵入性和活体成像均可检测 GFP 表达的原位膀胱癌，两种方法的相关性 r=0.99。
结论
这是首个非侵入性荧光成像的膀胱癌原位模型，可用于快速筛选这种难治性疾病的新型有效药物。
Zotero 插件