Demarest K, Hitzemann B, Phillips T, Hitzemann R
Department of Neurobiology and Behavior, SUNY at Stony Brook, New York 11794-8101, USA.
Alcohol Clin Exp Res. 1999 Jan;23(1):87-95.
The effect of ethanol on the number of Fos-like immunoreactive (Fos-li) neurons was previously studied in the C57BL/6J (B6) and DBA/2J (D2) inbred mouse strains (Hitzemann and Hitzemann, 1997). Data obtained suggested that the locomotor activation response to ethanol found in the D2 but not the B6 strain was associated with an increase in the number of Fos-li neurons (a putative measure of synaptic activity) in the central nucleus of the amygdala (CeA), but not in other brain regions, including the basal ganglia. Supporting results were obtained in B6D2 F2 intercross animals (Demarest et al., 1998) those animals exhibiting a marked locomotor activation response to ethanol also showed a significant increase in the number of Fos-li neurons in the CeA. The current study extends this line of investigation to the FAST and SLOW selected lines of mice (Shen et al., 1995). Twenty-eight SLOW and FAST mice (taken evenly from both replicate lines) were randomly assigned to receive either saline or ethanol (1.5 g/kg). One hour later, the animals were sacrificed, and the number of Fos-li neurons were determined using standard immunocytochemical techniques. Both the FAST and SLOW lines showed a marked increase (>300%) in the number of Fos-li neurons in the lateral aspect of the CeA; however, in the capsular division, only the FAST line showed an increase (>500%). In several brain regions, the basal (saline) response was markedly higher in the SLOW line; these regions included the subthalamic nucleus, the entopeduncular nucleus, the substantia nigra compacta, and the ventral tegmental area. Furthermore, it was found that ethanol decreased the number of Fos-li neurons in the ventral tegmental area of the SLOW but not FAST mice. These data suggest a substantial involvement of the basal ganglia in the segregation of the FAST and SLOW lines.
先前在C57BL/6J（B6）和DBA/2J（D2）近交系小鼠品系中研究了乙醇对Fos样免疫反应性（Fos-li）神经元数量的影响（希茨曼和希茨曼，1997年）。获得的数据表明，D2品系而非B6品系中发现的对乙醇的运动激活反应与杏仁核中央核（CeA）中Fos-li神经元数量（一种假定的突触活动测量指标）的增加有关，而在包括基底神经节在内的其他脑区则未发现这种增加。在B6D2 F2杂交动物中也获得了支持性结果（德马雷斯特等人，1998年），那些对乙醇表现出明显运动激活反应的动物在CeA中Fos-li神经元数量也显著增加。当前的研究将这一研究方向扩展到了快速和慢速选择的小鼠品系（沈等人，1995年）。28只慢速和快速小鼠（从两个重复品系中平均选取）被随机分配接受生理盐水或乙醇（1.5克/千克）。一小时后，处死动物，使用标准免疫细胞化学技术确定Fos-li神经元的数量。快速和慢速品系在CeA外侧区域的Fos-li神经元数量均显著增加（>300%）；然而，在被膜分区中，只有快速品系显示出增加（>500%）。在几个脑区，慢速品系的基础（生理盐水）反应明显更高；这些区域包括丘脑底核、内苍白球核、黑质致密部和腹侧被盖区。此外，还发现乙醇减少了慢速而非快速小鼠腹侧被盖区的Fos-li神经元数量。这些数据表明基底神经节在快速和慢速品系的分离中起重要作用。
