节肢动物中参与细胞质不亲和或性别比例畸变的沃尔巴克氏体菌株的间隔区2区域和5S核糖体DNA变异

Spacer 2 region and 5S rDNA variation of Wolbachia strains involved in cytoplasmic incompatibility or sex-ratio distortion in arthropods.

作者信息

van Meer M M, van Kan F J, Stouthamer R

机构信息

Laboratory of Entomology, Wageningen, The Netherlands.

出版信息

Lett Appl Microbiol. 1999 Jan;28(1):17-22. doi: 10.1046/j.1365-2672.1999.00476.x.

DOI:10.1046/j.1365-2672.1999.00476.x

PMID:10030026

Abstract

Bacteria in the genus Wolbachia are widespread in arthropods and can induce sex-ratio distortion or cytoplasmic incompatibility in their hosts. The phylogeny of Wolbachia has been studied using 16S rDNA and the cell cycle gene ftsZ, but sequence variation of those genes is limited. The spacer 2 region (SR2) was amplified to determine whether this region would improve phylogenetic resolution. The SR2 of Wolbachia is 66 bp long, shows higher variation than ftsZ and has very low homology with closely related bacteria. Due to the small length of SR2 of Wolbachia, little phylogenetic information could be retrieved.

摘要

沃尔巴克氏体属细菌在节肢动物中广泛存在，可在其宿主中诱导性别比例畸变或细胞质不亲和。人们已利用16S rDNA和细胞周期基因ftsZ对沃尔巴克氏体的系统发育进行了研究，但这些基因的序列变异有限。扩增间隔区2区域（SR2）以确定该区域是否能提高系统发育分辨率。沃尔巴克氏体的SR2长66 bp，变异程度高于ftsZ，与亲缘关系密切的细菌同源性极低。由于沃尔巴克氏体SR2长度较短，可检索到的系统发育信息很少。